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Method for fermenting lactic acid bacteria

A technology of lactic acid bacteria fermentation and lactic acid, which is applied in the direction of fermentation, etc., can solve the problems of long production cycle, inability to eliminate, and inability to eliminate the feedback inhibition of cellulase, and achieve the effects of short enzymatic hydrolysis time, release of inhibition, and ensuring food safety

Active Publication Date: 2011-07-27
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the fermentation of lactic acid by straw mostly adopts the step-by-step enzymatic hydrolysis and saccharification fermentation method: cellulase enzymatic hydrolysis and lactic acid fermentation are carried out step by step. The disadvantage is that the production cycle is long, and the glucose and cellobiose produced in the enzymatic hydrolysis process cannot be eliminated. Feedback inhibition, cannot eliminate the glucose effect of high concentration of glucose generated during enzymatic hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Slant culture: Inoculate lactic acid bacteria (Lactobacillus 1.2131) (purchased from Institute of Microbiology, Chinese Academy of Sciences) into the slant medium under aseptic conditions, and culture at 30° C. for 40 hours.

[0026] 2) Shake flask seed culture: Inoculate the lactic acid bacteria strains obtained in step 1) into the seed liquid culture medium, and perform anaerobic culture at 40° C. and 200 rpm for 20 hours.

[0027] 3) Fermentation culture: Inoculate the lactic acid bacteria seeds obtained in step 2) into the lactic acid bacteria fermentation broth for cultivation, the inoculation amount is 10% (v / v), 40°C, 200rpm, anaerobic culture.

[0028] Wherein, the culture medium method that step 1) uses is:

[0029] Straw hydrolyzed sugar 1wt%, yeast extract 0.5wt%, peptone 0.5wt%, agar 2wt%, prepared in tap water.

[0030] Wherein, the medium formula used in step 2) is:

[0031] Straw hydrolyzed sugar 3wt%, yeast extract 0.5wt%, peptone 0.15wt%, corn stee...

Embodiment 2

[0036] 1) Slant culture: Inoculate lactic acid bacteria (Lactobacillus 1.2131) (purchased from Institute of Microbiology, Chinese Academy of Sciences) into the slant medium under aseptic conditions, and culture at 30° C. for 40 hours.

[0037] 2) Shake flask seed culture: Inoculate the lactic acid bacteria strains obtained in step 1) into the seed liquid culture medium, and perform anaerobic culture at 40° C. and 200 rpm for 20 hours.

[0038] 3) Fermentation culture: Inoculate the lactic acid bacteria seeds obtained in step 2) into the lactic acid bacteria fermentation broth for cultivation, the inoculum amount is 10% (please select a fixed value) (v / v), 40°C, 200rpm, anaerobic culture.

[0039] Wherein, the culture medium method that step 1) uses is:

[0040] Straw hydrolyzed sugar 1wt%, yeast extract 0.5wt%, peptone 0.5wt%, agar 2wt%, prepared in tap water.

[0041] Wherein, the medium formula used in step 2) is:

[0042] Straw hydrolyzed sugar 3wt%, yeast extract 0.5wt%,...

Embodiment 3

[0047] 1) Slant culture: Inoculate lactic acid bacteria (Lactobacillus 1.2131) (purchased from Institute of Microbiology, Chinese Academy of Sciences) into the slant medium under aseptic conditions, and culture at 30° C. for 40 hours.

[0048] 2) Shake flask seed culture: Inoculate the lactic acid bacteria strains obtained in step 1) into the seed liquid culture medium, and perform anaerobic culture at 40° C. and 200 rpm for 20 hours.

[0049] 3) Fermentation culture: Inoculate the lactic acid bacteria seeds obtained in step 2) into the lactic acid bacteria fermentation broth for cultivation, the inoculation amount is 10% (v / v), 40°C, 200rpm, anaerobic culture.

[0050] Wherein, the culture medium method that step 1) uses is:

[0051] Straw hydrolyzed sugar 1wt%, yeast extract 0.5wt%, peptone 0.5wt%, agar 2wt%, prepared in tap water.

[0052] Wherein, the medium formula used in step 2) is:

[0053] Straw hydrolyzed sugar 3wt%, yeast extract 0.5wt%, peptone 0.15wt%, corn steep ...

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Abstract

The invention relates to a method for fermenting lactic acid bacteria. The method comprises the steps of: utilizing celluloses of plant straws, which are treated with partial enzymolysis, as a carbon source for the fermentation of lactic acid bacteria so as to produce lactic acid by fermentation, and causing the sugar content of celluloses of plant straws, which are treated with partial enzymolysis, to range from 1 to 10 in percentage by weight; and partial enzymolysis comprises the steps of: carrying out enzymolysis on the celluloses of plant straws for 12 to 72 hours through cellulose at the temperature from 45 to 55 DEG C so that sugar generated through enzymolysis and celluloses which are not treated with the enzymolysis can be contained in fermentation liquor and the lactic acid can be prepared through enzymolysis and fermentation simultaneously. Compared with the prior art, the production cycle is shortened to 64 hours from 136 hours, the inhibition of high-concentration glucose on lactic acid bacteria fermentation lactic acid is gotten rid off, and the fermentation level is increased to 6.8 from 3.6 in percentage by weight. The method utilizes the plant straws as raw materials for fermentation, makes waste profitable, reduces environmental pollution and has positive significance to guarantee food security.

Description

technical field [0001] The invention relates to a technology for fermenting and producing lactic acid by using plant straw cellulose, in particular to a method for fermenting and producing lactic acid by using plant straw cellulose as a carbon source after partial enzymatic hydrolysis. Background technique [0002] Lactic acid is an important organic acid. Lactic acid, lactate and its derivatives have been widely used in food, feed, medicine, leather, printing and dyeing, chemical industry and other fields. It is widely used as sour agent, preservative and reducing agent in food industry. In recent years, in order to eliminate the environmental crisis caused by the "white pollution" caused by plastic products, poly L-lactic acid, a bioengineering material chemically synthesized with L-lactic acid as a monomer, has been developed. Degradable plastics can be completely biodegraded and absorbed, eventually forming carbon dioxide and water, without polluting the environment. S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56
Inventor 李荣杰尚海涛崔刚
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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