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Method for preparing polystyrene micro-sphere micro-array by ultrasonic focusing micro-jet process

A technology of polystyrene microspheres and ultrasonic focusing, which is applied to the process for producing decorative surface effects, microstructure technology, and microstructure devices. Promote the use and other issues to achieve the effect of reducing the number, improving positioning accuracy, and reducing production costs

Inactive Publication Date: 2011-07-06
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned polystyrene microsphere microarray has problems such as high production cost, complicated process, difficult maintenance of biological activity, easy clogging of sampling needles or nozzles, and large amount of reagents, which are not conducive to popularization and use.

Method used

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  • Method for preparing polystyrene micro-sphere micro-array by ultrasonic focusing micro-jet process
  • Method for preparing polystyrene micro-sphere micro-array by ultrasonic focusing micro-jet process
  • Method for preparing polystyrene micro-sphere micro-array by ultrasonic focusing micro-jet process

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Embodiment Construction

[0036] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific diagrams.

[0037] refer to figure 1 , figure 2 , image 3 , Figure 4 , Figure 5 A method for preparing a polystyrene microsphere microarray by ultrasonic focusing micro-spraying comprises the following steps: the first step is to prepare a micro-spraying reagent: preparing a polystyrene (PS) microsphere suspension solution. According to the difference of polystyrene microsphere aggregation numbers in single spraying point 5, the mass fraction of polystyrene microsphere suspension solution used in microspraying can be prepared arbitrarily according to needs. The present invention preferably adopts a suspension solution of polystyrene microspheres with a mass fraction of 10%. The particle size range of the polystyrene microspheres used for micro-spraying ...

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Abstract

The invention relates to the field of preparing micro-array biochips, in particular to a method for preparing a polystyrene micro-sphere micro-array by an ultrasonic focusing micro-jet process. The method comprises the following steps: preparing a suspension of polystyrene micro-spheres; cleaning a reagent reservoir, pouring the suspension of polystyrene micro-spheres into the reagent reservoir and ensuring that an ultrasonic focusing micro-jet nozzle is in a jet-ready state; taking a micro-array substrate, clamping the micro-array substrate on a micro-displacement bench, and arranging the micro-displacement bench below an ultrasonic focusing micro-jet device; sending an operating command; sending a jet command to make individual jet points of the suspension of polystyrene micro-spheres; setting a row distance, a column distance and a jet point number of the micro-array; making a polystyrene micro-sphere micro-array; and stamping and fixing the polystyrene micro-sphere micro-array on a flat plate after the polymerization of poly(dimethylsiloxane) (PDMS). With the technical scheme, the method can achieve the purpose of nozzle-free, non-blocking, non-contact and low-consumption preparation of polystyrene micro-sphere micro-arrays.

Description

technical field [0001] The invention relates to the field of microarray biochip preparation, in particular to a method for preparing polystyrene microsphere microarray. Background technique [0002] With the maturity of the preparation technology of monodisperse and controllable particle size polymer microspheres, the application of polymer microspheres has become more and more extensive, especially the monodisperse polystyrene microspheres with a particle size of 2-30 μm. The application value of the ball is the highest. Polystyrene microspheres are used as the carrier, on which various functional groups or functional materials such as active proteins, nucleic acids, and magnetic metals can be loaded, and the polystyrene microspheres loaded with functional groups or functional materials can be arrayed or patterned, which can be It is used in the fields of medicine, bioengineering, food chemical industry, information engineering and microelectronics technology. Especially ...

Claims

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Application Information

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IPC IPC(8): B81C1/00
Inventor 韩鑫张德远张波王娟
Owner SHANDONG UNIV OF TECH
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