Feed yeast culture additives, preparation method and application thereof for high producing dairy cows
A technology of yeast culture and dairy cow feed, which is applied in the field of production of yeast culture additives for high-yield dairy cow feed, can solve problems such as application limitations, and achieve the effects of reducing the number of protozoa, promoting mass reproduction and proliferation, and enhancing full digestion and absorption
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Embodiment 1
[0029] The production method of the high-yielding milk cow feed yeast culture additive comprises: common brewer's yeast saccharomycescerevisiae, the rejuvenation of brewer's yeast by introducing the brewer's yeast under the condition of aseptic operation technique, and carrying out under the condition of aseptic operation Brewer's yeast is expanded and cultivated, fermented, and dried to make finished products through a granulator.
Embodiment 2
[0031] The preparation method of the high-yield milk cow feed yeast culture additive described in embodiment 1, the rejuvenation of described bacterial classification: the test tube bacterial classification that preserves is transferred to the test tube that the sterilized solid medium is housed using aseptic technique cultured at a constant temperature of 30°C for 3-5 days; stored at 4°C, and transplanted once every 4-5 months.
[0032] The expanded culture of the strains: under the condition of aseptic operation, carry out the expanded culture of brewer's yeast, inject sterile 0.9% physiological saline into the cultured test tube, shake it to make a bacterial suspension, and then inoculate it into a 1L-capacity In an Erlenmeyer flask filled with 300ml of liquid medium, culture in a constant temperature shaking incubator at 30°C and 180r / min for 36h.
Embodiment 3
[0034] In the preparation method of the high-yield milk cow feed yeast culture additive described in embodiment 1 or 2, the solid medium is prepared by first mixing corn stalks, brewer's grains and cornmeal in a ratio of 20:75:5, and then mixing them The mixture was pulverized to 60-80 mesh, then fully mixed with water at a ratio of 1:1, put into a sterilizing pot at 121°C for 30 minutes, and finally taken out and cooled in a natural state.
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