Additive for reducing methane release of ruminant rumen
A ruminant and additive technology, applied in animal feed, animal feed, drug combination, etc., can solve problems such as reducing feed digestibility
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Embodiment 1
[0038] The effects of additives on methane, rumen fermentation environment, and the number of cellulolytic bacteria were tested in vitro and in vivo.
[0039] According to the test, 200mg containing 70% hay, 18% corn, 6.5% soybean cake, 2.5% cottonseed cake, 2% premix, 1% salt are uniformly ground and mixed through a 40-mesh analytical sieve, and then added to the fermentation bottle . Add the additives in the preferred proportions of the present invention to the bottles containing the above-mentioned feed mixes in triplicate.
[0040] Three sheep were fed the ground feed and 500 ml of coarsely filtered rumen fluids were mixed with 1000 ml of colemans simplex. The buffer has the following composition:
[0041] colemans simplex slow liquid
[0042]a Trace element solution: Dissolve CaCl2.2H2O 13.2g, MnCl2.4H2O 10.0g, CoCl2.6H2O 1.0g, FeCl3.6H2O 8.0g with distilled water, cool, and dilute to 100ml.
[0043] b Buffer solution: NaHCO3 35.0g, NH4HCO3 4.0g, dissolve in distilled...
Embodiment 2
[0062] The additive extract of the preferred formulation of the present invention was blended with the additive amount of 1.5% in Example 1, and the effects of feeding sheep in the feed on methane production, rumen internal environment indicators and the number of fibrinolytic bacteria. Twelve 1.5-year-old Karakul sheep weighing about 30 were randomly divided into two groups. The experimental animals were housed in well-ventilated metabolic cages, single cages, and equipped with automatic drinking water devices. The animals were fed at 5:30 in the morning and 5:30 in the afternoon, and had free access to water throughout the day. The feed intake and remaining feed of the experimental sheep were recorded every day, and samples were taken to determine the dry matter intake. According to Johnson et al. (1994) SF6 tracer technology to measure the amount of methane production, while collecting rumen fluid to measure the rumen environment index and the number of fibrinolytic bacteri...
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