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Extraction method of astaxanthin and ornamental fish feed with astaxanthin

An extraction method and technology of astaxanthin, applied in the field of ornamental fish farming, can solve the problem that astaxanthin is difficult to absorb and utilize by ornamental fish, and achieve the effect of strengthening physical fitness

Inactive Publication Date: 2011-06-29
湖州唐氏渔业科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the feed of edible fish cannot be directly used for the breeding of ornamental fish.
Astaxanthin is a natural pigment that can enhance the color of ornamental fish; if the astaxanthin-rich shrimp and crab shell powder is directly used as a feed additive, the astaxanthin in it is difficult to be absorbed and utilized by ornamental fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The extraction method of astaxanthin comprises the following steps:

[0027] ① After drying the shrimp and crab shells, soak them in acetic acid until softened, then wash and dry;

[0028] ②Crush the softened and dried shrimp and crab shells, pass through a 150-mesh sieve, and then use protease hydrolysis to remove protein under the condition of pH value of 2-3 and temperature of 40-45°C;

[0029] ③ Add dichloromethane to the deproteinized shrimp and crab shell powder, and process it with 2000-3000MHz ultrasonic wave for 10-30min, and control the temperature at 30-35°C, and filter to obtain the crude astaxanthin extract;

[0030] ④ suction filter the astaxanthin crude extract, and the filtrate is heated in a vacuum water bath to remove residual methylene chloride to obtain solid astaxanthin coarse powder;

[0031] ⑤ Dissolve astaxanthin coarse powder with ethanol, and then use hydrochloric acid to precipitate astaxanthin.

[0032] A feed for ornamental fish, comprisin...

Embodiment 2

[0038] The extraction method of astaxanthin comprises the following steps:

[0039] ① After drying the shrimp and crab shells, soak them in acetic acid until softened, then wash and dry;

[0040] ②Crush the softened and dried shrimp and crab shells, pass through a 150-mesh sieve, and then use protease hydrolysis to remove protein under the condition of pH value of 2-3 and temperature of 40-45°C;

[0041] ③ Add dichloromethane to the deproteinized shrimp and crab shell powder, and process it with 2000-3000MHz ultrasonic wave for 10-30min, and control the temperature at 30-35°C, and filter to obtain the crude astaxanthin extract;

[0042] ④ suction filter the astaxanthin crude extract, and the filtrate is heated in a vacuum water bath to remove residual methylene chloride to obtain solid astaxanthin coarse powder;

[0043] ⑤ Dissolve astaxanthin coarse powder with ethanol, and then use hydrochloric acid to precipitate astaxanthin.

[0044] A feed for ornamental fish, comprisin...

Embodiment 3

[0050] The extraction method of astaxanthin comprises the following steps:

[0051] ① After drying the shrimp and crab shells, soak them in acetic acid until softened, then wash and dry;

[0052] ②Crush the softened and dried shrimp and crab shells, pass through a 150-mesh sieve, and then use protease hydrolysis to remove protein under the condition of pH value of 2-3 and temperature of 40-45°C;

[0053] ③ Add dichloromethane to the deproteinized shrimp and crab shell powder, and process it with 2000-3000MHz ultrasonic wave for 10-30min, and control the temperature at 30-35°C, and filter to obtain the crude astaxanthin extract;

[0054] ④ suction filter the astaxanthin crude extract, and the filtrate is heated in a vacuum water bath to remove residual methylene chloride to obtain solid astaxanthin coarse powder;

[0055] ⑤ Dissolve astaxanthin coarse powder with ethanol, and then use hydrochloric acid to precipitate astaxanthin.

[0056] A feed for ornamental fish, comprisin...

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Abstract

The invention relates to an extraction method of astaxanthin and ornamental fish feed with astaxanthin, belonging to the field of ornamental fish culturing technology. The extraction method of astaxanthin comprises the following steps: (1) drying shrimp and crab shells, soaking the shell with acetic acid until the shells are softened, cleaning the softened shells and drying; (2) crushing and removing protein with protease; (3) preparing coarse extraction liquid of astaxanthin; (4) preparing coarse powder of astaxanthin; and (6) dissolving the coarse powder of astaxanthin with ethanol, and precipitating the astaxanthin with hydrochloric acid. The ornamental fish feed is characterized by comprising the following substances in percentage by weight: 5-15% of astaxanthin, 20-50% of animal protein powder and the balance of binder. The ornamental fish feed provided by the invention is complete in nutrition and rich in natural pigment--astaxanthin, and can provide comprehensive nutrition as well as pure natural astaxanthin for the ornamental fish body; and moreover, the astaxanthin not only can make the entire fish body bright and glossy, but also has anti-oxidation effect as well as remarkable effect on improving the fish constitution.

Description

technical field [0001] The invention relates to an extraction method of astaxanthin and an ornamental fish feed added with astaxanthin, belonging to the technical field of ornamental fish breeding. Background technique [0002] Compared with natural bait, artificial feed has the following advantages: First, the source is relatively stable and can be produced continuously throughout the year, while natural bait is easily restricted by seasons; second, it can be produced according to different stages of fish growth and development. The feed formula can be adjusted at any time for different nutritional needs, which not only meets the nutritional needs of fish at different stages, but also reduces the cost of breeding; third, a certain amount of drugs can be added according to seasonal changes to prevent and treat some common fish diseases; fourth, it can be passed through high temperature Treatment can kill some pathogenic bacteria. The body color of ornamental fish is signifi...

Claims

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Application Information

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IPC IPC(8): A23K1/10A23K1/18A23K10/20A23K10/22A23K20/179A23K50/80C07C403/24
Inventor 陈再忠唐文彬潘泓宇
Owner 湖州唐氏渔业科技开发有限公司
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