Pretreatment method of extraction of target product in fermentation liquor

Abstract

The invention provides a pretreatment method of the extraction of a target product in fermentation liquor. The pretreatment method comprises three steps, namely sieve filtration, micro-pipe bundle settling and ultrafiltration. The pretreatment method is as follows: firstly, the fermentation liquor is filtered by a sieve to obtain suspension 1; secondly, the particulate matter with higher settlingvelocity in the suspension 1 is separated through micro-pipe bundle settling to obtain extract 2; and finally, the residual fine particles and macromolecular substances in the extract 2 are removed through ultrafiltration, and the obtained clear liquid is sent to a follow-up membrane device to separate and extract the target product. In the pretreatment method of the invention, micro-pipe bundle settling is adopted to replace filtration including microfiltration, centrifugation and flocculation before the ultrafiltration membrane, thus effectively removing the particulate matter in the fermentation liquor and reducing the blockings of the membrane in the follow-up membrane treatment. Therefore, the cleaning frequency of the membrane can be lowered and the service life of the membrane can be prolonged. The method of the invention has low cost, is easy to operate, is safe and effective and is a fermentation liquor pretreatment method suitable for the industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of industrial microbial fermentation engineering, and in particular relates to a pretreatment method for extracting a target product from a fermentation liquid. Background technique [0002] The fermentation process is a complex microbial growth and metabolism process. Along with the metabolic process, a variety of primary and secondary metabolites will be produced, making the composition of the fermentation broth complex, such as cell fragments produced by cell self-melting and the cells themselves, and microbial metabolism. Sugars, protein colloids, and incompletely utilized residual substances in the medium are often only a part of the fermentation broth components for the target products we need. Therefore, in order to extract and purify the target product, it is first necessary to separate and remove various broken microbial cells, colloids such as sugar and protein, and solid impurities such as fiber p...

AI Technical Summary

Problems solved by technology

[0004] Installing microfiltration units (including microporous tube filtration units) before ultrafiltration is an effective method to prevent ultrafiltration membrane clogging and reduce the frequency of ultrafiltration membrane cleaning, but it will also bring problems such as increased investment costs for pretreatment equipment

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