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Method for producing total flavonoids of chrysanthemum

A production method and technology of total flavonoids, which are applied in pharmaceutical formulas, medical preparations containing active ingredients, food science, etc., can solve problems affecting product quality and clinical curative effect, poor quality controllability, large dosage, etc., and achieve improvement Clinical use, suitable for large-scale production, and the effect of improving the preparation level

Inactive Publication Date: 2011-06-22
安徽协和成药业饮片有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are very few chrysanthemum preparations on the market, most of which are limited by the production process, contain more impurities, take large doses, and have poor quality controllability, which directly affect product quality and clinical efficacy.

Method used

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  • Method for producing total flavonoids of chrysanthemum
  • Method for producing total flavonoids of chrysanthemum
  • Method for producing total flavonoids of chrysanthemum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The best extraction method

[0034] Choose four factors for water extraction and alcohol extraction, with 3 levels for each factor. Take 5g of Bo chrysanthemum and test it according to the L9(34) orthogonal table. The extraction rate of total flavonoids in medicinal materials is used as an evaluation index to screen the best extraction process.

[0035] The level arrangement of water extraction factors is shown in Table 1, the orthogonal test results are shown in Table 2, and the variance analysis results are shown in Tables 3 and 4.

[0036] Table 1 Water extraction factor level table

[0037]

[0038] Table 2 Orthogonal test results of water extraction

[0039]

[0040] Table 3 Results of analysis of variance in water extraction (extraction rate of total flavonoids)

[0041]

[0042] F0.01(1.2)=99; F0.05(1,2)=19

[0043] Table 4 Results of analysis of variance by water extraction (extract yield)

[0044]

[0045] F0.01(1.2)=99; F0.05(1,2)=19

[0046] According to the in...

Embodiment 2

[0065] Example 2: Optimal separation and purification method

[0066] Compare AB-8 type (weak polarity), D101 type (non-polar), DA201 type (polar), DM301 (medium polarity), HPD600 (polar) 5 kinds of different polar macroporous resins to Bo The adsorption properties of total flavonoids in chrysanthemum.

[0067] Weigh 30g each of various types of resins that have been pretreated, and apply wet method to the column (φ1.5cm×40cm). Precisely absorb 80ml of the loading solution (equivalent to 0.1g crude drug / ml) for adsorption on the column and pre-adsorb for 1h. The effluent from the column is re-adsorbed once to make the adsorption complete. The effluent is collected, washed with distilled water until there is no reducing sugar reaction, and then used 10% and 30%. Elute with 50%, 70%, and 90% ethanol, and collect the respective eluates. Determine the concentration of total flavonoids and total saponin to calculate the specific adsorption capacity and specific elution capacity. Test...

Embodiment 3

[0072] Take 500g of Bo chrysanthemum, add 10 times the amount of 70% ethanol, reflux and extract 2 times, 2h each time, combine the extracts, recover the ethanol until there is no alcohol smell, centrifuge, take the supernatant, and dilute with distilled water to a concentration of 4.61mg / ml (equivalent to 0.1g crude drug / ml), spare;

[0073] Load the solution obtained in step (1) on the AB-8 macroporous adsorption resin column. The concentration of the sample solution is 4.61mg / ml (equivalent to 0.25g crude drug / g resin), and the sample is adsorbed at a rate of 1BV / h. The impurities were eluted with 10BV distilled water, and then eluted with 4BV / h 50% ethanol at a flow rate of 2BV / h. The 50% ethanol eluate was collected, the solvent was recovered, and dried under reduced pressure to obtain the total flavonoids of Bo Chrysanthemum.

[0074] Perform the test in 3 parallel. Determine the content of total flavonoids, the amount of dry paste, calculate the transfer rate, yield and pr...

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Abstract

The invention relates to a method for producing total flavonoids of chrysanthemum. The method comprises the following steps of: extracting crude extracts from the chrysanthemum, and purifying the crude extracts to obtain the total flavonoids of the chrysanthemum. The method is characterized in that: the macroporous resin which is AB-8 type macroporous resin is used for purification. The content of the total flavonoids of the chrysanthemum, prepared by the method, is over 50 percent, nearly reaches 70 percent; the total flavonoids are high in purity and yield; moreover, the method is environmentally-friendly and applicable to industrial mass production.

Description

Technical field [0001] The invention discloses a method for producing the total flavonoids of Bo Chrysanthemum. It belongs to the pharmaceutical field. Background technique [0002] Chrysanthemum is the dried flower head of the chrysanthemum Dendrathema morifolium (Ramat.) of the compositae family. It is a commonly used traditional Chinese medicine in my country with sweet and bitter taste. Return to lung and liver channel. The 2010 edition of the Pharmacopoeia includes Chuju, Boju, Gongju and Hangju, among which Boju and Chuju are authentic medicinal materials in Anhui Province. Chrysanthemum has been cultivated in my country for at least 1,500 years. Its edible and medicinal use has been recorded in the "Shen Nong's Materia Medica" as early as: "Long-term use promotes vitality, lightness, resistance to aging, and prolonged life." The "Materia Medica Biology" says: "Chrysanthemum has both sweet and bitter taste, calm temperament, suffering from the four qi, suffering from fros...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/287A23L1/30A61P9/10A61P9/12A61P39/06
Inventor 吴德玲金传山陈慧芳李素亮
Owner 安徽协和成药业饮片有限公司
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