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Bacterium detection method and device

A technology of bacteria detection and detection device, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems affecting the accuracy of the detection results of bacteria concentration in water samples, prolong the growth time of bacteria culture, etc., so as to facilitate the instrumentation and automation, achieving instrumentation and automation, improving accuracy and reliability

Inactive Publication Date: 2011-06-15
FOCUSED PHOTONICS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing method, the water sample is not separated and treated, but directly cultured and tested. The aforementioned chemical substances will inhibit the proliferation of bacteria, thereby prolonging the growth time of bacterial culture, and ultimately affecting the accuracy of the water sample bacterial concentration test results.

Method used

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  • Bacterium detection method and device
  • Bacterium detection method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Such as figure 1 , 2 , Shown in 3, a kind of bacterium detecting device comprises filter 11, culture unit 2, detection unit and analysis unit 4;

[0040] The filter 11 includes a filter membrane 111 and a connector 112, the filter membrane 111 is a hydrophilic polysulfone filter membrane with an equivalent filter pore size of 0.2um;

[0041] The sample with pressure (0.1-3 bar) enters the filter 11 through the connector 112, and the filtrate is discharged from the filter holes of the filter membrane 111 under the action of the pressure, and the bacteria in the sample are trapped on the inner wall of the filter membrane 111 ; The filter 11 is used to filter and accumulate bacteria in the sample to be tested, and the sample is a water sample in this embodiment;

[0042] The culture unit 2 includes a culture container 211 and an incubator 212, and the culture container 211 is used to hold a culture solution containing a specific reaction substrate; since the present embo...

Embodiment 2

[0068] see Figure 4 , a kind of bacterium detection device, differs from the detection device described in embodiment 1: the detection device of this embodiment also includes a sealed bag and a refrigerator, and the filter 12 after sampling is preserved during transportation;

[0069] The filter membrane 121 is a hydrophilic Teflon filter membrane with an equivalent filter aperture of 0.1um;

[0070] The light emitting module 321 of the detection unit can emit light with a wavelength of 365nm, and the light receiving module 322 can detect light with a wavelength of 450nm; the culture container 221 is a colorless and transparent polycarbonate material, which is transparent to the emitted light and received light of the detection unit;

[0071] During the measurement, the culture container 221 is taken out from the incubator 212 and put into the measurement optical path of the detection unit arranged outside the culture unit, and the culture solution in the bacterial culture pr...

Embodiment 3

[0085] see Figure 5 , Figure 6 , a bacteria detection device, different from the detection device described in embodiment 1: the detection device also includes a driving pump 5, and the driving pump 5 will drive the sample into the filter 11;

[0086] The detection unit and analysis unit 4 are arranged in the incubator; the light emitting module 311 and the light receiving module 312 are arranged on opposite sides of the culture container 211 in the incubator 212, capable of emitting and detecting light with a wavelength of 405nm.

[0087] This embodiment also provides a kind of bacterial detection method, and the detection method difference described in embodiment 1 is:

[0088] Adopt the bacteria detection device described in this embodiment;

[0089] In step a, a 1.5L sample is collected from the field and brought back to the laboratory for bacterial retention and accumulation of the sample:

[0090] Adopt drive pump 5 to drive the sample collected from the scene, make...

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Abstract

The invention relates to a bacterium detection method which comprises the following steps: a. sampling: passing a sample to be detected through a filter, so that bacteria in the sample are accumulated in the filter; and metering the total volume of the sample passing through the filter; and b. culturing and detecting: putting the filter accumulated with the bacteria into a culture fluid, so that the bacteria are restricted in the filter to grow, reproduce and metabolize, and the metabolite generated by target bacteria and a substrate in the culture fluid are subjected to specific reaction; and detecting the information of the culture fluid to obtain the information of the target bacteria in the detected sample. The invention also provides a bacterium detection device. The invention has the advantages of accurate detection result and the like, is convenient for sampling, and can eliminate the interference of the coexisting substances.

Description

technical field [0001] The invention relates to sample detection, in particular to a method and device for detecting bacteria in a sample. Background technique [0002] Surface water, ground water, and even rainwater contain many types of bacteria. When the water body is polluted by human and animal feces, domestic sewage or some industrial and agricultural wastewater, the bacteria in the water body will increase significantly. Therefore, the bacteriological examination of water, especially the examination of intestinal bacteria, is of great significance in hygiene. By examining the number of specific types of bacteria in the water, the hygienic quality of the water can be judged. [0003] In the existing methods for the detection of bacteria in water bodies, the large-volume water samples collected on-site are generally brought back to the laboratory for analysis. Since there is a certain time interval from sampling to detection in this method, the following conditions n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
Inventor 项光宏
Owner FOCUSED PHOTONICS
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