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Chlorella strain and application thereof

A kind of technology of Chlorella, yl-2cgmccno.4305

Inactive Publication Date: 2011-06-15
福建漳州鼎能生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing reports, the algae strains used generally have problems such as high requirements for culture conditions, low biomass and oil production, and unstable biodiesel conversion, which are difficult to implement.

Method used

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  • Chlorella strain and application thereof
  • Chlorella strain and application thereof
  • Chlorella strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the isolation and identification of Chlorella YL-2

[0022] 1. Sample collection

[0023] Water samples containing green algae were collected from Qinghe, Beijing in July 2010.

[0024] 2. Isolation and screening of Chlorella YL-2

[0025] Separation steps: Transfer the collected water samples to domestic sewage (taken from a city domestic sewage treatment plant) that has been autoclaved (121°C, sterilized by high-pressure steam for 20 minutes), and place them in a light incubator for enrichment and cultivation. After three rounds of enrichment culture, properly dilute the algae liquid and mix it with the sewage agar semi-solid medium, then culture under light, pick a single algae and drop it in a small test tube to expand the culture.

[0026] Screening steps: first inoculate the pure algae strains on a 48-well microplate (each hole contains 0.25mL of sterile sewage), place them in a light incubator for cultivation, detect the growth at 540nm, select th...

Embodiment 2

[0040] Embodiment 2, the determination of Chlorella YL-2 seed culture medium

[0041] 1. Selection of basal medium

[0042] Large-scale cultivation of microalgae using sewage requires a certain amount of good seeds, so the culture medium (BG11 medium, SE medium, Shuisheng No. -2 growth effects.

[0043] 1. Preparation of culture medium

[0044] (1) BG11 medium

[0045] Prepare BG11 medium (pH 7.0).

[0046] BG11 medium (pH7.0) made of NaNO 3 、K 2 HPO 4 ·3H 2 O, MgSO 4 ·7H 2 O, CaCl 2 2H 2 O, citric acid, ferric ammonium citrate, Na 2 EDTA, Na 2 CO 3 、A 5 +Co solution and distilled water; each liter of medium contains 1ml A 5 +Co solution, 1.5g NaNO 3 , 0.04g K 2 HPO 4 ·3H 2 O, 0.075g MgSO 4 ·7H 2 O, 0.036g CaCl 2 2H 2 O, 0.006g citric acid, 0.006g ferric amine citrate, 0.001gNa 2 EDTA and 0.02g Na 2 CO 3 .

[0047] A 5 +Co solution consists of solute and water; solute and its concentration are: 2.86g / L H 3 BO 3 , 1.81g / L MnCl 2 ·H 2 O, 0.222g / L...

Embodiment 3

[0075] The growth situation of embodiment 3, chlorella YL-2

[0076] The raw sewage was taken from a domestic sewage treatment plant in a certain city. After sedimentation, it was not sterilized. The water quality conditions are shown in Table 1.

[0077] Table 1 Raw sewage water quality

[0078] water quality index

Index value

Total Nitrogen (mg / L)

2091.63

Ammonia nitrogen (mg / L)

349.09

Total phosphorus (mg / L)

84.2

CODcr (mg / L)

3502

pH

6.9

[0079] 1. Cultivation of Chlorella YL-2

[0080] Cultivate Chlorella YL-2 to the logarithmic growth phase (OD) in the seed medium prepared in Example 2 at 28°C 540nm About 0.8), transfer to the Erlenmeyer flask with 20% (volume ratio) (500ml of raw sewage in each 1000ml Erlenmeyer flask), and cultivate for 7 days under the conditions of 28°C, light intensity 1000~1200lux, and light-dark ratio 14h:10h ( growth stagnation).

[0081] 2. Determination of bioma...

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Abstract

The invention discloses a Chlorella strain and an application thereof. The Chlorella strain provided by the invention is Chlorella sp. YL-2 of which collection number is CGMCC No.4305. The Chlorella sp. YL-2 can well grow in sewage, and can efficiently remove nitrogen and phosphorus in sewage. The Chlorella sp. YL-2 is rich in oil and fat, and can be used for preparing biodiesel. Therefore, the Chlorella sp. YL-2 has wide application prospects in culture using sewage and biodiesel conversion system.

Description

technical field [0001] The invention relates to a strain of chlorella and its application, in particular to the application of the chlorella in the application fields of bioenergy and sewage purification. Background technique [0002] The energy crisis and the water resource crisis are two major crises facing the world at present. According to relevant reports, the global mineral and fossil energy reserves: 150 billion tons of oil, with a service life of 41 years; 550 billion tons of coal, with a service life of 200 years; 110 billion tons of natural gas, with a service life of 60 years. With the rapid development of social economy and the continuous increase of population, limited fresh water resources have been seriously polluted and destroyed, and the world is facing a severe crisis of water shortage. Therefore, the development of new renewable bioenergy (biodiesel) and new technologies for biological advanced treatment of sewage is a powerful measure to deal with the in...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64
CPCY02E50/13Y02E50/10
Inventor 黄三福聂新峰王海胜闫艳春李信
Owner 福建漳州鼎能生物科技有限公司
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