Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for culturing cordyceps militaris sporocarp with high-content bioactive substances

A technology of biologically active substances and cultivation methods, applied in the field of new resource food, can solve the problems that do not involve the increase of the yield and content of biologically active substances in fruiting bodies of Cordyceps militaris

Inactive Publication Date: 2013-02-13
陈卫东 +2
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the invention patent with the application number of 03135295.2 "A method for increasing the content of cordycepin", the invention patent with the application number of 200610117998.9 "the industrial cultivation method of Cordyceps militaris with high content of cordycepin", the invention with the application number of 200810068949.X Patent "Method for increasing cordycepin content in Cordyceps militaris solid medium", application number 01124109.8 Invention patent "Method for producing cordycepin by fermentation culture (liquid culture)", application number 200810068734. Invention patent "Monascus Synergistic Fermentation Method for Improving the Yield of Cordyceps Militaris Fruiting Bodies and Cordycepin", the invention patent application number 200310101650.7 "Application of 3'-deoxyadenosine in the Preparation of Blood Lipid-lowering Drugs", etc. However, these invention patents mainly focus on Cordyceps militaris In the study of the method of improving the yield of fruiting bodies and cordycepin production, there is no research on the increase of the yield of the whole biologically active substance and its content in the fruiting body of Cordyceps militaris

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 12°C±1°C, the humidity is at 65%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 20°C, and the relative humidity should be 70%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturi...

Embodiment 2

[0018]In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 14°C±1°C, the humidity is at 70%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 21°C, and the relative humidity should be 75%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturit...

Embodiment 3

[0020] In the preparation of the culture medium, the weight share of its various fractions is: choose about 30 grams of rice with a particle size of 12 mesh-20 mesh (accounting for 41% of the total weight), and 30 milliliters of the solution configured in step 2. After calculation, the content of carbon element is 0.47%, and the content of nitrogen is 0.034%. Inject the liquid Cordyceps militaris into the container containing the above-mentioned medium under aseptic conditions. During the vegetative growth period, the ambient temperature of the strain is kept at 16°C±1°C, the humidity is at 75%, and it is protected from light within the first five days. , give scattered light irradiation for the last five days; in the late growth stage, the ambient temperature should be kept at about 22°C, and the relative humidity should be 80%. At the same time, 0.5% glucose solution is sprayed on the surface of the medium to supplement the carbon source. After entering the growth and maturi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for culturing cordyceps militaris sporocarp with high-content bioactive substances, comprising the following steps: inoculating good cordyceps militaris strains after multiple repeated filtering on a culture medium with the best optimized proportion of carbon to nitrogen sources; and optimizing and culturing at different temperatures, humidity and illumination intensities at different growth periods. Through the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the contents of bioactive substances are greatly improved, and the bioactive substances mainly comprise the chemical index components of adenosine, cordycepin, cordyceps polysaccharide, cordycepic acid, SOD (Super Oxide Dlsmutase) and the like; for the cordyceps militaris sporocarp planted and obtained by the method provided by the invention, the pharmacology and drug effect are extremely similar to natural Chinese caterpillar fungus, and the contents of bioactive substances of adenosine, cordycepin, cordyceps polysaccharide and cordycepic acid are over that of the natural Chinese caterpillar fungus.

Description

Technical field: [0001] The invention belongs to the technical field of new resource food. In particular, it relates to a method for industrialized cultivation of fruit bodies of Cordyceps militaris with high content of biologically active substances. It particularly relates to an industrialized cultivation method for increasing the content of biologically active substances in fruiting bodies of Cordyceps militaris. Background technique: [0002] Natural wild Cordyceps sinensis is formed in a special ecological and extreme environment. It has special genes and expression products and traits, has strict polytrophy, and requires a special ecological and geographical environment in alpine regions. Therefore, the yield is very small. In 2005, the country banned the digging of wild Cordyceps, so the contradiction between supply and demand is very prominent now. In order to alleviate the contradiction between supply and demand, my country has listed the artificial cultivation of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G1/00
Inventor 陈卫东张华陈春保陈腊梅
Owner 陈卫东
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com