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Engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in northeast China region

A straw cellulose and regional technology, applied in the field of fungi that degrade cellulose, can solve problems such as waste of material resources, and achieve the effects of improving utilization rate, high enzyme activity, and efficient degradation ability.

Inactive Publication Date: 2012-06-27
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The world produces nearly 200 billion tons of crop straw every year, and our country produces more than 600 million tons every year. A large number of agricultural residues are produced every year, most of which are not fully utilized and discarded as waste, which not only brings serious environmental problems , wasting valuable material resources

Method used

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  • Engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in northeast China region
  • Engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in northeast China region
  • Engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in northeast China region

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specific Embodiment approach 1

[0011] Specific Embodiment 1: In this embodiment, an indigenous fungus with high enzyme activity and efficient ability to degrade straw cellulose in Northeast China, the fungus is named Penicillium decumbens C5, belongs to the genus Penicillium sp., and has been Preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation number is CGMCC No.4203, and the preservation date is October 8, 2010; its hyphae have septa, conidiophores are smooth, mostly oval or spherical, 1.0 μm to 1.5 μm in diameter, 2 to 5 asymmetric branches, arranged closely, in broom shape (such as figure 1 shown), with 5 to 10 short bottle-shaped stalks (10.0 μm to 15.0 μm in size) growing on its top, in the form of unbranched chains (such as figure 2 Shown); form a round colony on the culture medium, with white hyphae on the edge, rough surface, covered with a layer of conidia powder, grayish green, and the back of the colony is tea-brown.

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specific Embodiment approach 2

[0019] Embodiment 2: This embodiment differs from Embodiment 1 in that the growth temperature of Penicillium decumbens C5 is -4°C to 35°C, and the growth pH is between 5.5 and 7.5. Others are the same as in the first embodiment.

[0020]In this embodiment, Penicillium decumbens C5 is cultivated with improved Cha's medium. As an indigenous fungus in Northeast China, its growth temperature is -4°C to 35°C, and its growth pH is between 5.5 and 7.5.

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Abstract

The invention relates to an engonus fungus with high enzyme activity and high capacity of efficiently degrading straw cellulose in the northeast China region, in particular to a fungus for degrading cellulose. The engonus fungus with high enzyme activity and high capacity of efficiently degrading the straw cellulose in the northeast China region is named as Penicillium decumbens C5, belongs to the Penicillium sp., and has the preservation number of CGMSS No.4203 and the preservation date of October 8th 2010. When the Penicillium decumbens C5 strain is cultured for 3d, FPase reaches 7085.66U / L, the EG / Cen peak is 3856.97U / L, the CBU / Cex peak is 2681.59U / L, and the beta-Gase peak is 1487.86U / L, thus the Penicillium decumbens C5 has a function of efficiently degrading straws.

Description

technical field [0001] The invention relates to a fungus strain degrading cellulose. Background technique [0002] Cellulose is the oldest and most abundant natural polymer on the earth, and it is the most precious natural renewable resource for human beings. The world produces nearly 200 billion tons of crop straw every year, and our country produces more than 600 million tons every year. A large number of agricultural residues are produced every year, most of which are not fully utilized and discarded as waste, which not only brings serious environmental problems , but also a waste of valuable material resources. Corn stalks are agricultural residues with a high content of cellulose components. How to make corn stalks harmless and recycle has become a research hotspot at home and abroad. The use of cellulase produced by microorganisms to hydrolyze straw has the advantages of stable effect and less pollution, so it has been widely recognized and respected. Therefore, scr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/80
Inventor 高大文李保深梁红
Owner NORTHEAST FORESTRY UNIVERSITY
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