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Light control method for dendrobium officinale tissue culture

A Dendrobium candidum and tissue culture technology, applied in the field of bioengineering, can solve the problems that the emission spectrum of fluorescent lamps can not meet the selective demand for light for the growth of Dendrobium candidum, and the long seedling cultivation period, so as to shorten the cultivation period and reduce the Production cost, effects of stable genetic traits

Active Publication Date: 2011-05-18
浙江森宇药业有限公司
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AI Technical Summary

Problems solved by technology

However, since the emission spectrum of fluorescent lamps cannot well meet the selective demand for light in different growth stages of Dendrobium candidum, it is one of the main reasons for the long cultivation period of Dendrobium candidum seedlings.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Use 1 / 2MS medium as the basic medium as the seed germination medium. After the preparation is completed, put it into a test tube for sterilization and disinfection for use;

[0012] Take the fruit of Dendrobium officinale, sterilize it with 70-75% ethanol solution for 8 minutes, then remove it from the ethanol solution and place it on sterile filter paper, cut the fruit, shake off the powdered seeds into the seed germination medium, and put the inoculated The culture medium is placed in an incubator with an LED (semiconductor light-emitting diode) plant growth lighting system as the light source. The light quality is white light, 10-15 μmol m -2 ·s -1 The light intensity and light time are 12 hours / day, and the culture temperature is 25±2°C. After the embryo germinates to form a protocorm, it grows into a 1-2cm high seedling after about 2-3 months of cultivation.

[0013] Transfer the test tube seedlings that have grown to 1-2cm to the seedling growth medium (the basi...

Embodiment 2

[0015] Use 1 / 2MS medium as the basic medium as the seed germination medium. After the preparation is completed, put it into a test tube for sterilization and disinfection for use;

[0016] Take the fruit of Dendrobium officinale, sterilize it with 70-75% ethanol solution for 8 minutes, then remove it from the ethanol solution and place it on sterile filter paper, cut the fruit, shake off the powdered seeds into the seed germination medium, and put the inoculated The culture medium is placed in an incubator with an LED (semiconductor light-emitting diode) plant growth lighting system as the light source. The light quality is white light, 10-15 μmol m -2 ·s -1 The light intensity and light time are 12 hours / day, and the culture temperature is 25±2°C. After the embryo germinates to form a protocorm, it grows into a 1-3cm high seedling after about 2-3 months of cultivation.

[0017] Transfer the test tube seedlings that have grown to 2-3cm to the seedling growth medium (the basi...

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Abstract

The invention relates to a light control method for dendrobium officinale tissue culture, which comprises the following steps of: obtaining powdery seeds after dendrobium officinale fruits are sterilized, culturing the powdery seeds through a seed germination culture medium, and germinating the cultured seeds to form protocorms. The method is characterized in that: in the tissue culture process of dendrobium officinale seedlings, a light emitting diode (LED) plant growth illumination system is used as a light source, and different illumination treatment is implemented according to different stages of dendrobium officinale tissue culture. The different illumination treatment is implemented according to different growth stages so that the dendrobium officinale seedlings can furthest utilize light energy for photosynthesis and the highest growth amount is obtained. Experimental results show that: the culture period of the dendrobium officinale seedlings can be effectively shortened according to the technical scheme, the time from the seeds to the rooted finished seedlings is only 150 to 180 days, the growth period is shortened by 30 to 60 days compared with the culture under a common fluorescent lamp, and the seedlings cultured by the method have stable hereditary character and good quality.

Description

technical field [0001] The invention relates to a light regulation method for tissue culture of dendrobium candidum seedlings, belonging to the technical field of bioengineering. Background technique [0002] Dendrobium officinale Kimura et Migo is a traditional traditional Chinese medicine, which has the effects of nourishing yin and clearing heat, nourishing qi and promoting body fluid, relieving cough and moistening lung, clearing throat and brightening the moon. Dendrobium contains various chemical components such as polysaccharides and alkaloids. Modern pharmacological studies have proved that the medicinal activity of Dendrobium is related to the amount of polysaccharides. Dendrobium polysaccharides can enhance the body's immune function and significantly improve the body's ability to kill tumor cells. It is a regulator of tumor immunotherapy. [0003] At present, the cultivation of Dendrobium officinale seedlings is mainly carried out in the form of tissue culture in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 斯金平黄华宏朱玉球潘学冬周泓谢彬彬
Owner 浙江森宇药业有限公司
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