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Method for producing organophosphorus pesticide degrading bacterium by utilizing sweet potato starch wastewater

A technology of sweet potato starch wastewater and organophosphorus pesticides, applied in the biological field

Inactive Publication Date: 2011-05-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production method of organophosphorus pesticide degrading bacteria agent produced by sweet potato starch wastewater has not yet been found in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Production of organophosphorus pesticide degrading bacterial agent

[0022] (1) Medium preparation:

[0023] 2) Strain preservation medium (solid, 1L): 2g peptone, 1g yeast extract, 5g sodium chloride, 20g agar, add sweet potato starch wastewater to 1L, pH 7.0-7.5;

[0024] 5) Strain activation medium (liquid, 1L): peptone 2g, yeast extract 2g, sodium chloride 5g, add sweet potato starch wastewater to 1L, pH 7.0-7.5;

[0025] 6) Seed medium (liquid, 10L): MgSO 4 1.0g, KH 2 PO 4 5.0g, NaCl 5.0g, K 2 HPO 4 15.0g, peptone 10g, add sweet potato starch wastewater to 10L, pH 7.0-7.5;

[0026] 7) Fermentation medium (liquid, 200L): MgSO 4 20g, KH 2 PO 4 100g, NaCl 100g, K 2 HPO 4 300g, (NH 4 ) 2 SO 4 0.8kg, add sweet potato starch wastewater to 200L, pH 7.0-7.5;

[0027] All the above media were sterilized at 121°C for 15 min.

[0028] 2. Strain activation: pick organophosphorus pesticide-degrading bacteria to the strain preservation medium,...

Embodiment 2

[0032] Example 2: Degradation effect of organophosphorus pesticide degrading bacteria agent on dichlorvos pesticide residues on rape leaves

[0033] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 80% dichlorvos EC pesticide;

[0034] 2) After 12 hours of spraying dichlorvos, the pests have been killed, and the pesticide degrading bacteria agent (diluted 600 times with water) is evenly sprayed, and the spraying of clean water is used as a control;

[0035]3) After 24 hours of spraying the fungicide, the five-point sampling method was used to detect the residue of dichlorvos on the leaves of rapeseed. It was found that compared with the control group without spraying the fungicide, the residual amount of dichlorvos in the treatment group sprayed with the fungicide was significantly reduced, which was lower than that of the countries with residual dichlorvos in vegetables. Limit standard (0.1mg / kg).

Embodiment 3

[0036] Embodiment 3: Degradation effect of organophosphorus pesticide degrading bacteria agent on rape leaf dimethoate

[0037] 1) Select two identical rape plots, each 30m 2 , evenly spray 1000 times diluted 50% Dimethoate EC pesticide;

[0038] 2) After 12 hours, evenly spray the pesticide-degrading bacterial agent (diluted 600 times with water), and use spraying water as a control;

[0039] 3) After 48 days, the five-point sampling method was used to detect the residues of dimethoate on rapeseed leaves, and it was found that the residues of dimethoate in the treatment group were significantly lower than those in the control group, which was lower than the national limit standard for residues of dimethoate in vegetables.

[0040] The bacterial strain that degrades organophosphorus pesticides provided by the present invention is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation date is Decembe...

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Abstract

The invention relates to a method for producing a high-efficiency bacillus cereus organophosphorus pesticide degrading bacterium by utilizing sweet potato starch wastewater, which belongs to the technical field of environmental biology. The organophosphorus pesticide degrading bacterium adopts a strain of bacillus cereus separated from the surfaces of rape leaves. The production method for the bacterium comprises the five steps of: the preparation of a culture medium, strain activation, the preparation of liquid seeds, liquid state fermentation and the preparation of the bacterium. A main carbon source and water for the fermentation of the culture medium are provided by the sweet potato starch wastewater. In the technical scheme provided by the invention, a new way is provided for the utilization of the high-concentration sweet potato starch wastewater as resources, and the produced organophosphorus pesticide degrading bacterium can highly-efficiently degrade organophosphorus pesticide residues on leaves of plants in situ so as to ensure food safety and protect the ecological environment.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for producing organic phosphorus pesticide highly efficient degrading bacterial agent by using sweet potato starch wastewater as the main raw material. technical background [0002] Organophosphorus pesticides are still the most used category of pesticides because of their many varieties, high efficacy, and broad-spectrum control. They play a pivotal role in agricultural production. The production and use of organophosphorus pesticides in my country account for More than half of the total production and use of pesticides. However, organophosphorus pesticides are highly toxic. After long-term and large-scale use, their residues on crops will cause food safety problems and affect the export of agricultural products in my country. Moreover, scattered pesticides will also pollute water and soil. [0003] The bioremediation method using microorganisms to degrade organophosp...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02C12R1/085A62D101/04A62D101/26
Inventor 彭霞薇荆梦姜丹宿燕明安晓宇白志辉
Owner BEIJING FORESTRY UNIVERSITY
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