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Method for preparing sinigrin extract

A technology of sinoside and extract is applied in the field of preparation of active ingredients in plants, and achieves the effects of simple process, easy operation and avoiding oxidation

Active Publication Date: 2011-05-11
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the preparation of sinigrin extract from cruciferous plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The preparation method of embodiment 1 sinigrin extract, comprises the following steps:

[0015] 1) Take the dried mustard seeds, pulverize them, boil and heat-preserve them for 2 hours with ethanol aqueous solution equivalent to 8 times the weight of the raw materials, 60% (v / v), and extract for 2 hours, filter, and then use 60% (v / v) ethanol solution equivalent to 10 times the weight of the raw materials / v) extracted with ethanol aqueous solution for 1 hour, filtered, combined extracts, centrifuged, and collected supernatant;

[0016] 2) Add absolute ethanol to the supernatant until the concentration of ethanol in the mixed solution is 80% (by mass), leave it for 1 hour, centrifuge, and collect the secondary centrifuged solution;

[0017] 3) The secondary centrifugal liquid is concentrated until it has no alcohol smell, and the obtained concentrated centrifugal liquid is passed through a 0.5 μm ceramic membrane, and the permeate is concentrated to twice the weight of...

Embodiment 2

[0020] The preparation method of embodiment 2 sinigrin extract comprises the following steps:

[0021] 1) Take the dried cabbage seeds, crush them, boil them with 80% (v / v) ethanol aqueous solution equivalent to 10 times the weight of the raw materials, and extract for 2 hours, filter, concentrate the extract to 4 times the weight of the raw materials, centrifuge, and collect Serum;

[0022] 2) Concentrate the supernatant to 0.5 times the weight of the raw material, add 95% (v / v) aqueous ethanol solution therein until the ethanol concentration in the mixed solution is 85% (mass), leave it for 1 hour, centrifuge, and collect two Secondary centrifugal fluid;

[0023] 3) The secondary centrifugal liquid is concentrated until there is no alcohol smell, and the obtained concentrated centrifugal liquid is passed through a 0.5 μm ceramic membrane, and the permeate is concentrated to 3 times the weight of the raw material to obtain the concentrated permeate;

[0024] 4) Concentrate ...

Embodiment 3

[0026] The preparation method of embodiment 3 sinigrin extract, comprises the following steps:

[0027] 1) Take the stems and leaves of fresh mustard greens, crush them to 20-30 meshes, boil and heat-preserve them for 2 hours with an aqueous ethanol solution equivalent to 3 times the weight of the raw materials and 70% (v / v), filter, and use the residue equivalent to the weight of the raw materials 2 times, 80% (v / v) ethanol aqueous solution extraction for 2 hours, filter, combine the extracts, concentrate the extracts to 4 times the weight of the raw materials, take out and let cool, then centrifuge, and collect the supernatant;

[0028] 2) Concentrate the supernatant to 1.5 times the weight of the raw material, then add 90% (v / v) aqueous ethanol to it until the ethanol concentration in the mixture is 82% (by mass), place it for 1.5 hours, centrifuge, and collect Secondary centrifugal fluid;

[0029] 3) The secondary centrifugal liquid is concentrated until there is no alcoh...

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PUM

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Abstract

The invention discloses a method for preparing a sinigrin extract. The method comprises the following steps of: 1) extracting crucifer brassica mustard leaf, cabbage or broccoli serving as a raw material by using acetic acid, and centrifuging the extract to obtain supernate; 2) adding ethanol into the supernate, adjusting the ethanol concentration to be 80 to 85 percent, standing the solution for 1 to 1.5 hours, centrifuging the solution, and collecting secondary centrifugate; 3) concentrating the secondary centrifugate till no ethanol smell, filtering the concentrated centrifugate by using a ceramic film of 0.5 micron, concentrating the permeating solution until the volume of the permeating solution is 2 to 3 times weight of the raw materials to obtain concentrated permeating solution; and 4) filling the concentrated permeating solution into a macroporous resin column, collecting the effluent, eluting the effluent by using water in an amount which is 2 to 3 times weight of the resin, collecting eluant, combining the effluent and the eluant, and performing concentration and freeze drying to obtain the sinigrin extract. The method has simple process and is easy to operate, the sinigrin content of the obtained product can reach 20 to 40 percent, and the extraction rate of the sinigrin reaches over 80 percent.

Description

technical field [0001] The invention relates to a preparation method of active components in plants, in particular to a preparation method of sinigrin extract. Background technique [0002] Back in the 1880s, an international committee recommended eating more cruciferous vegetables for their cancer protection. Cruciferous plants in my country mainly include cauliflower, broccoli, cabbage, radish, and mustard greens. Among the cruciferous plants, Brassica is the main edible vegetable. Brassica plants include broccoli, cauliflower, turnip, roasted green cabbage, kale, mustard greens and so on. Epidemiological data have shown that a diet rich in cruciferous vegetables such as broccoli, cauliflower, and kale reduces the incidence of many cancers. At present, it is agreed that the precursors of the important anticancer active ingredients of cruciferous plants are Glucosinolates. More than 120 different glucosinolates have been found in natural plants, and they exist in 11 diff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/31A61K31/7028A61P35/00
Inventor 宋云飞梁远盛赵军孙步祥汤凌志
Owner GUILIN NATURAL INGREDIENTS CORP
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