Kit for inducing differentiation from bone mesenchymal stem cells to fat cells, application of kit and method for inducing cell differentiation

Abstract

The invention provides a kit and method for inducing differentiation from rat bone mesenchymal stem cells to fat cells. The kit comprises a fat cell inducing culture solution and a fat cell identification solution, wherein the inducing culture solution comprises a cell culture solution A, a cell culture solution B, a cell culture solution C, a cell culture solution D and a cell culture solution E; the fat cell identification solution comprises a cell immobilizing solution, a coloring agent, a detergent A and a detergent B; the cell culture solution A is an alpha-minimum essential medium (MEM) liquid culture medium; the cell culture solution B is fetal bovine serum; the cell culture solution C is a dexamethasone solution; the cell culture solution D is a 1-methyl-3-isobutylxanthine solution; the cell culture solution E is an insulin solution; the cell immobilizing solution is a paraformaldehyde solution; the coloring agent is an oil red O solution; the detergent A is a 60% isopropanol solution; and the detergent B is double distilled water.

Description

technical field [0001] The present invention relates to a kit for inducing cell differentiation and its application and a method for inducing cell differentiation, in particular to a kit for inducing differentiation of bone marrow mesenchymal stem cells to fat cells and its application and method for inducing bone marrow mesenchymal stem cells A method for the differentiation of stem cells into fat cells. Background technique [0002] Bone marrow mesenchymal stem cells are a type of adult stem cells with multi-directional differentiation potential, which can be differentiated into adipocytes, chondrocytes, adipocytes, nerve cells, liver cells and other tissue cells, which can be applied clinically in tissue engineering and gene therapy , cytokine replacement therapy and other fields. [0003] Bone marrow mesenchymal stem cells are a group of cells with unique phenotypes. Due to the lack of specific surface markers, how to determine whether the obtained cell clones are bone ...

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Problems solved by technology

However, studies have found that bone marrow mesenchymal stem cells can be induced to differentiate into adipocytes for as long as 3-4 weeks, which adds uncertainty and risk to the experiment

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