Complex microorganism organic fertilizer and preparation method thereof
A technology of compound microorganisms and organic fertilizers, which is applied in the preparation of organic fertilizers, the treatment of biological organic parts, organic fertilizers, etc., which can solve the problems of smaller living space of beneficial microorganisms, weakened disease resistance, cold resistance, drought resistance, and decline in crop quality, etc. problem, to reduce the occurrence of crop disease (pest) damage, enhance the ability of disease (pest) resistance, promote crop growth and improve the effect of
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Embodiment 1
[0027] The culture preparation of embodiment 1 yeast
[0028] 1) Activation of strains: insert the yeast parent species into the slant activation medium for activation. The weight percentage of each component in the slant culture medium is: glucose 1.0%, peptone 1.0%, magnesium sulfate 0.2%, potassium dihydrogen phosphate 0.3%, dipotassium hydrogen phosphate 0.5%, agar 2.0%, add water to 100% ; Adjust the pH value to 5.6, and sterilize at 1.21KPa for 20 minutes.
[0029] Culture environment: culture temperature 28~30℃; culture time 48 hours.
[0030] 2) Primary seed culture: (based on 20Kg)
[0031] Inoculate the activated yeast into the seed medium in an amount of 5%, and the seed tank medium is composed of: 2Kg brown sugar, 40g magnesium sulfate, 60g potassium dihydrogen phosphate, 100g dipotassium hydrogen phosphate, add water to 20Kg; adjust pH The value is 5.6-7.0, and it is boiled to sterilize.
[0032] Culture environment: culture temperature 28~30℃; culture time 48...
Embodiment 2
[0036] The culture preparation of embodiment 2 bacillus lateral sporosum
[0037]1) Activation of strains: the parent species of Bacillus lateralis was inserted into the slant activation medium for activation. The percentage by weight of each component in the slant culture medium is: peptone 1.0%, beef extract 0.3%, sodium chloride 0.5%, agar 2.0%, add water to 100%; adjust the pH value to 7.0, sterilize at 1.21KPa for 20 Minutes, slant streak culture, culture temperature 32 ~ 35 ℃, culture time 24 hours.
[0038] 2) Primary seed culture: (based on 20Kg)
[0039] Inoculate the activated Bacillus sporogenes in the seed culture medium in an amount of 5%, and the culture medium is composed of: 202g of soybean flour, 200g of corn flour, 100g of sodium chloride, 100g of potassium dihydrogen phosphate, and adding water to 20Kg; adjust the pH value 7.0, boiled to sterilize.
[0040] Culture environment: culture temperature 32~35℃; culture time 48 hours.
[0041] 3) Secondary seed...
Embodiment 3
[0044] The culture preparation of embodiment 3 bacillus megaterium
[0045] With the cultivation preparation method of Bacillus lateralis in embodiment 2
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