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Efficient rooting method for oil tea clone tissue culture seedlings

A technology for tissue culture seedlings and clones, which is applied to the field of rooting of tissue culture seedlings of Camellia oleifera clones, and can solve the problems of difficult rooting of tissue culture seedlings, difficulty in transplanting and survival, etc.

Active Publication Date: 2011-04-13
HUNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The documents retrieved above show that it is very difficult for tissue culture seedlings of Camellia oleifera to take root, and it is difficult to survive transplanting.

Method used

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  • Efficient rooting method for oil tea clone tissue culture seedlings
  • Efficient rooting method for oil tea clone tissue culture seedlings
  • Efficient rooting method for oil tea clone tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Cut the young shoots of the year from the robust plants of Camellia oleifera, and take the top 4--6 cm for disinfection; transfer the aseptic seedlings obtained in the first generation to WPM+BA3mg / L+IBA0.5mg / L+ZT0.05mg / L Subculture, the multiplication period is 40 days, and the multiplication coefficient reaches 4.2; the aseptic seedlings obtained in the multiplication process are strengthened by WPM+IBA1.0mg / L+biotin 0.5mg / L, and the tissue-cultured seedlings grow to 2- Take it out from the culture bottle at -4 cm, soak the base of the tissue culture seedling in 1000ppm IBA solution for 10 seconds, then inoculate it on the medium of 1 / 4MS+AC200, and the rooting rate reaches 89% after 30 days. After rooting, the tissue-cultured seedlings were moved to a nutrient cup, the substrate was peat soil:perlite=3:1, and the transplanting survival rate reached 93%.

[0024] The specific operation is as follows:

[0025] (1) Transfer the obtained sterile seedlings to the subcult...

Embodiment 2

[0029] Cut the young shoots of the year from the robust plants of Camellia oleifera, and take the top 4--6cm for disinfection; transfer the sterile seedlings obtained from the first generation to WPM+BA5mg / L+IBA2mg / L+ZT2mg / L for subculture, and the multiplication cycle After 30 days, the multiplication coefficient reached 9.1; the aseptic seedlings obtained in the multiplication process were strengthened by WPM+IBA0.1mg / L+biotin 2mg / L, and when the tissue cultured seedlings grew to 2--4 cm in 25 days, they were cultivated Take it out from the bottle, soak the base of the tissue culture seedling in 3000ppm IBA solution for 5 seconds, then inoculate it on the medium of MS+AC600, and the rooting rate reaches 91% after 25 days. After rooting, the tissue-cultured seedlings were moved to a nutrient cup, the substrate was peat soil:perlite=2:1, and the transplanting survival rate reached 91%.

[0030] See embodiment 1 for specific operations.

Embodiment 3

[0032] Cut the young shoots of the current year from the robust plants of Camellia oleifera, and take the top 4--6cm for disinfection; transfer the sterile seedlings obtained in the first generation to WPM+BA5mg / L+IBA0.1mg / L+ZT1mg / L for subculture, The multiplication cycle is 35 days, and the multiplication coefficient reaches 7.1; the sterile seedlings obtained during the multiplication process are strengthened by WPM+IBA2mg / L+biotin 3mg / L, and the tissue cultured seedlings grow to 2--3 cm in 25 days. Take it out from the bottle, soak the base of the tissue culture seedling in 4000ppm IBA solution for 5 seconds, then inoculate it on the medium of 1 / 2MS+AC400, and the rooting rate reaches 85% after 30 days. After rooting, the tissue-cultured seedlings were moved to a nutrient cup, the substrate was peat soil:perlite=1:1, and the transplanting survival rate reached 89%.

[0033] See embodiment 1 for specific operations.

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Abstract

The invention provides a process for quickly propagating a large quantity complete oil tea clone plants with roots by tissue culture, which comprises the steps of secondary culture medium screening and hormone combination, seedling strengthening culture medium screening and hormone combination, root preprocessing, and rooting culture medium screening and hormone combination, wherein WPM+BA (Woody Plant Medium and Benzyladenine, 3-7mg / L), IBA (Indol Butyric Acid, 0.1-2mg / L) and ZT (Zeatin, 0.05-2mg / L) are used as secondary culture media for propagation, the propagation period is shortened to 30-40 days, and the propagation coefficient reaches 4.2-9.1; WPM+IBA (0.1-2mg / L) and biotin (0.5-3mg / L) are used as seedling strengthening culture media for strengthening seedlings, and seedlings can take root after 20-25 days; and after being treated with IBA, tissue culture seedlings which are 2-4cm in height are directly inoculated on the culture media of MS (Murashige and Skoog medium, 1 / 4-1) and AC (Activated Carbon, 200-600), the tissue culture seedlings take root and form complete plants after 25-3 0days, and the rooting rate reaches more than 85%.

Description

technical field [0001] The invention relates to a rooting method for tissue-cultured seedlings of camellia oleifera clones, in particular to a method for obtaining a large amount of rooted camellia oleifera clone seedlings through rapid propagation through tissue culture. Background technique [0002] Camellia spp (Camellia spp) is a high-quality woody oil tree species unique to my country, and is known as the world's four largest woody oil tree species together with oil palm, coconut and olive [1] . Camellia oil extracted from Camellia oleifera seeds is rich in nutrients, with an unsaturated fatty acid content of over 90%, making it an ideal edible oil [2] , known as "Oriental Olive Oil" [3] . In addition, tea oil can be used industrially to produce oleic acid and its esters, to produce soap and vaseline, etc., and can also be used to produce stearic acid and glycerin; in medicine, it can be used to make injections and prepare various ointments, Pills, etc.; in the cosm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈永忠王晓明王瑞李永欣王湘南彭邵锋王玉娟蔡能曾慧杰马力杨小胡陈隆升
Owner HUNAN ACAD OF FORESTRY
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