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Method for extracting L-threonine

A technology for threonine and threonine fermentation broth, applied in the field of biochemical industry, can solve the problems of inconvenient operation, cumbersome steps, influence on the extraction purity of L-threonine, etc., and achieve the effects of simple steps and improved purity

Active Publication Date: 2011-04-06
梅花生物科技集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the fermentation broth needs to be decolorized, the steps are cumbersome, and the operation is inconvenient, and the mother liquor after crystallization is directly mixed with the fermentation broth without treatment, which affects the extraction purity of subsequent L-threonine

Method used

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  • Method for extracting L-threonine
  • Method for extracting L-threonine

Examples

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Effect test

Embodiment 1

[0032] Embodiment 1: the method for extracting L-threonine described in the present invention

[0033] 1. Extraction method

[0034] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25°C. The volume ratio of the fermentation broth is 1:4.

[0035] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is respectively controlled at 50°C, 60°C, 70°C, and 80°C, and the Baume concentration of the filtrate is finally controlled to reach 7, Then enter the single-effect evaporator for further concentration, control the temperature at 55°C, control the vacuum degree at -0.092Mpa, stir at 80rpm, concentrate until the solid-liquid ratio of the filtrate reaches 0.45:1, pu...

Embodiment 2

[0039] Embodiment 2: the method for extracting L-threonine described in the present invention

[0040] 1. Extraction method

[0041] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25°C. The volume ratio of the fermentation broth is 1:6.

[0042] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is respectively controlled at 50°C, 60°C, 70°C, and 80°C, and the Baume concentration of the filtrate is finally controlled to reach 8, Then enter the single-effect evaporator for further concentration, control the temperature at 58°C, control the vacuum degree at -0.092Mpa, stir at 80rpm, concentrate until the solid-liquid ratio of the filtrate reaches 0.75:1, put ...

Embodiment 3

[0046] Embodiment 3: the method for extracting L-threonine described in the present invention

[0047] 1. Extraction method

[0048] The L-threonine fermentation broth is sterilized and filtered through a ceramic membrane. The ceramic membrane has a pore size of 50nm, a molecular weight of 30KD, an operating pressure of 1.5bar, a transmembrane pressure difference of 1bar, and a temperature of 25°C. The volume ratio of the fermentation broth is 1:5.

[0049] The obtained L-threonine filtrate enters the four-effect evaporation for preliminary concentration, the vacuum degree is controlled at -0.092Mpa, the temperature is respectively controlled at 50°C, 60°C, 70°C, and 80°C, and the Baume concentration of the filtrate is finally controlled to reach 9, Then enter the single-effect evaporator for further concentration, control the temperature at 57°C, control the vacuum degree at -0.092Mpa, stir at 80rpm, concentrate until the solid-liquid ratio of the filtrate reaches 0.6:1, put...

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Abstract

The invention relates to the field of biochemical engineering, in particular to a method for extracting L-threonine. The method comprises the following steps of: (1) micro-filtrating L-threonine fermentation liquor, concentrating a filtrate to a solid-to-liquid ratio of 0.45-0.75 to 1 and centrifugating to obtain L-threonine crystals and mother liquor; (2) carrying out cross-adsorption on the mother liquor, then, eluting with ammonia with a pH value of 10-14 and collecting eluant from the start of elution to the Baume degree of 1; and (3) adding the L-threonine fermentation liquor to the eluant in the step (2), carrying out micro-filtration, concentrating the filtrate to the solid-to-liquid ratio of 0.45-0.75 to 1, centrifugating to obtain L-threonine crystals and mother liquor and repeating the step (2) to the mother liquor. The method of the invention is simple and convenient for operation without bleaching and can obtain high-quality L-threonine through separation by controlling the concentration times, and the crystallized mother liquor after the cross-adsorption is mixed and cycled with the L-threonine fermentation liquor to improve the purity of the subsequently extracted L-threonine.

Description

technical field [0001] The invention relates to the field of biochemical industry, and specifically provides a method for extracting L-threonine. Background technique [0002] Threonine, the scientific name is 2-amino-3-hydroxybutyric acid, the English name is Threonine Itaconic acid, and the molecular formula is C 4 h 9 o 3 N, its properties are white orthorhombic crystal or crystalline powder, odorless, slightly sweet taste, soluble in water, insoluble in ethanol, ether and chloroform. Threonine is one of the 20 kinds of amino acids that make up proteins. It has two asymmetric carbon atoms and can have 4 isomers. However, L-threonine exists naturally and has physiological effects on organisms. Since humans and animals cannot synthesize L-threonine themselves, they need to be ingested from food, so L-threonine is an essential amino acid, which is mainly used in food fortifiers, medicines, feed additives, etc. [0003] As an important food fortifier, L-threonine can stre...

Claims

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Application Information

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IPC IPC(8): C07C229/22C07C227/40
Inventor 钟秀茹王海雷龚华常利斌
Owner 梅花生物科技集团股份有限公司
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