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Corynebacterium glutamicum capable of producing succinic acid with high yield

A technology of Corynebacterium glutamicum and succinic acid, which is applied in the field of microorganisms, can solve the problems of affecting the quality of fermentation products and increasing the separation load, and achieves the effect of low cost, few by-products and single fermentation products

Inactive Publication Date: 2011-03-09
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of fermentation and production of succinic acid by wild Corynebacterium glutamicum, it is always accompanied by by-products L-lactic acid and acetic acid, which affects the quality of the fermentation product and increases the load of subsequent separation.

Method used

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  • Corynebacterium glutamicum capable of producing succinic acid with high yield
  • Corynebacterium glutamicum capable of producing succinic acid with high yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Plate medium: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, agar 20g / L, pH7.0.

[0034] Aerobic fermentation medium: glucose 20g / L, K 2 HPO 4 ·3H 2 O 1.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O0.4g / L, FeSO 4 ·7H 2 O 20mg / L, MnSO 4 ·H 2 O 20mg / L, urea 2.5g / L, biotin 100μg / L, vitamin B1200μg / L, initial pH 7.0. 5L fermenter with 3L liquid, sterilized at 115°C for 10 minutes.

[0035] The bacteria with the preservation numbers ATCC13032 and CGMCC No.3991 were first activated on a 30°C plate, and after 24 hours, two rings of well-grown bacteria were put into 30mL fermentation medium, cultivated at 30°C and 200rpm for 12h, and then according to 3% (v / v) Transfer the inoculation amount into 3L fermentation medium, pass air 1v / vm, ​​rotate at 500rpm, 30°C, after aerobic fermentation for 12h, transfer to anaerobic fermentation, pass CO 2 0.2v / vm, ​​rotation speed 200rpm, culture at 30°C. Add 20g / L glucose at the beginning of anaerobic fermentation and 12h after fermen...

Embodiment 2

[0037] Plate culture medium: with embodiment 1.

[0038] Aerobic fermentation medium: corn mash liquid 400mL / L (the sugar content of corn mash liquid is about 50g / L), K 2 HPO 4 ·3H 2 O 1.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O 0.4g / L, FeSO 4 ·7H 2 O 20mg / L, MnSO 4 ·H 2O 20mg / L, urea 2.5g / L, biotin 100μg / L, vitamin B1200μg / L, initial pH 7.0. 5L fermenter with 3L liquid, sterilized at 115°C for 10 minutes.

[0039] The preservation numbers are ATCC13032 and CGMCC No.3991 bacteria first activated strains on 30 ° C plate, 24 hours after two rings of well-grown bacteria into 30 mL fermentation medium, 30 ° C, 200 rpm culture for 12 h, and then according to 3% (v / v) Transfer the inoculation amount into 3L fermentation medium, pass air 1v / vm, ​​rotate at 500rpm, 30°C, after aerobic fermentation for 14h, transfer to anaerobic fermentation, pass CO 2 0.2v / vm, ​​rotation speed 200rpm, culture at 30°C. Add 20g / L glucose at the beginning of anaerobic fermentation and 12h after...

Embodiment 3

[0041] Plate culture medium: with embodiment 1

[0042] Aerobic fermentation medium: glucose 30g / L, peptone 5g / L, FeSO 4 ·7H 2 O 20mg / L, MnSO 4 ·H 2 O20mg / L, initial pH7.0. 5L fermenter tank liquid volume 3L, sterilized at 115°C for 10 minutes.

[0043] The bacteria with the preservation numbers ATCC13032 and CGMCC No.3991 were first activated on a 30°C plate, and after 24 hours, two rings of well-grown bacteria were put into 30mL fermentation medium, cultivated at 35°C and 200rpm for 12h, and then according to 3% (v / v) Transfer the inoculation amount into 3L fermentation medium, pass air 1v / vm, ​​rotate at 500rpm, 30°C, after aerobic fermentation for 16h, transfer to anaerobic fermentation, pass CO 2 0.2v / vm, ​​rotation speed 200rpm, culture at 35°C. Add 20g / L glucose at the beginning of anaerobic fermentation and 12h after fermentation respectively. After 36 hours of fermentation, the fermentation broth of CGMCC No. contained 35.1 g / L succinic acid. The conversion ra...

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Abstract

The invention discloses a corynebacterium glutamicum capable of producing succinic acid with high yield. The systematic nomenclature of the corynebacterium glutamicum is corynebacterium glutamicum SA001 and the corynebacterium glutamicum is preserved in the China General Microbiological Culture Collection Center with the preservation number of CGMCC No.3991. The invention further discloses a method of producing the succinic acid by utilizing the corynebacterium glutamicum capable of producing the succinic acid with high yield. Compared with the chemical methods, the method disclosed by the invention has the advantages of low energy consumption and less pollution; and in the method, non-fossil resources are used as raw materials, and precious metals are not required to be used as catalysts, thus the cost is low, the process can be controlled easily and the yield is high. Compared with the original bacterium, the bacterial strain provided by the invention has the advantages of single fermentation product, less by-products and high yield.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a high-yield succinic acid Corynebacterium glutamicum. Background technique [0002] Succinic acid, also known as succinic acid, is a common natural organic acid. As the terminal product of the reducing end of the tricarboxylic acid cycle, it widely exists in the human body, animals, plants and microorganisms, and plays an important role in biological metabolism. Succinic acid can not only be used as an additive in food, medicine and other industries, but also can be used as a C4 platform compound for the synthesis of bulk chemicals such as 1,4-butanediol and tetrahydrofuran, as well as PBS (polybutylene succinate), etc. biodegradable material. [0003] The preparation methods of succinic acid mainly include chemical synthesis and biological methods. Compared with chemical methods that are difficult to control, low yield, low purity, and expensive catalysts, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/46C12R1/15
Inventor 郝宁严明许晟李艳谢红翠郝思清欧阳平凯
Owner NANJING UNIV OF TECH
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