Screening method and application of alpha-glucosidase inhibitor

Abstract

The invention belongs to the field of traditional Chinese medicines. The screening method of an alpha-glucosidase inhibitor comprises the following steps of: (1) establishing in vitro screening models; establishing in vitro screening models of alpha-glucosidase and a substrate PNPG (Para Nitrobenzene-Beta-D-Galactose Pyranoside); (2) establishing a component library: selecting traditional Chinesemedicines, systematically extracting each traditional Chinese medicine with various (more than two kinds) solvents according to a similar compatible principle according to the polarities from low to high, wherein multiple extracting parts can be obtained for each traditional Chinese medicine by adopting the method; and (3) screening: blending all the extracting parts of the traditional Chinese medicines into a solution with a certain concentration, adding the solution to a reaction model, and calculating the inhibition rate. The screening method is simple, reasonable and easy to operate and can be used for finally screening out traditional Chinese medicines and traditional Chinese medicine components having obvious inhibition effects on the alpha-glucosidase. In addition, the screened traditional Chinese medicines and the traditional Chinese medicine components can be used for developing products aiming to inhibit the alpha-glucosidase and particularly used for developing drugs for reducing the blood sugar and has industrialization significance.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, relates to a screening method for active components of traditional Chinese medicines inhibiting α-glucosidase, and also relates to the application of traditional Chinese medicine extracts. Background technique [0002] With the improvement of people's living standards and the change of dietary structure, the number of diabetic patients increases rapidly and shows a trend of younger age. Diabetes mellitus (DM) has become one of the major chronic diseases that seriously threaten human health. The two important indicators of diabetes detection are fasting blood sugar level and postprandial blood sugar level. Epidemiological studies have shown that the first symptom in the onset of diabetes is often postprandial hyperglycemia, and postprandial hyperglycemia is more likely to cause than fasting blood sugar. Various complications are also an important factor in the development of type Ⅱ dia...

AI Technical Summary

Problems solved by technology

At present, the in vitro screening methods for α-glucosidase inhibitors mainly include p-nitrophenyl-α-D-glucopyranoside (PNPG) method, glucose oxidase method and immobilized enzyme method, but most of them use spectrophotometry As a detection method, the consumption of reagents is large and the reproducibility is poor. Especially for the screening of complex samples or samples with pigment interference, the false positives are high, and some even have no activity in vivo at all.

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