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Biological chip for detecting specific glycoprotein, antibody or antigen by surface plasmon resonance technology

A surface plasmon and technical detection technology, which is applied in the detection field of ion resonance principle, can solve problems such as inability to glycosylate, and achieve the effect of simple and easy method, high specificity and sensitivity, and simplified operation steps

Active Publication Date: 2011-02-09
上海睿昂基因科技股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Eukaryotic genes expressed in prokaryotes cannot be glycosylated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Embodiment 1 Utilizes biochip to detect liver cancer

[0072] 1. Laying gold film

[0073] 1.1 Prepare gold sol by trisodium citrate reduction method: take 100mL of 0.01% chloroauric acid aqueous solution and heat to boil, add 0.75ml of 1% trisodium citrate aqueous solution while stirring, and the golden yellow chloroauric acid aqueous solution turns into Purplish red, continue to boil for 15 minutes, make up to the original volume with distilled water after cooling.

[0074] 1.2 Gold film laying:

[0075] 1.2.1 After the slides are acid-washed, put them in N-β-(aminoethyl)-γ-aminopropyl trivalent oxysilane (APTMS) / toluene solution, reflux for 12 hours, wash them, take them out, and blow them with nitrogen Dry, store in a clean and dry place for later use;

[0076] 1.2.2 After soaking the treated glass slides in the nano-gold solution for 12 hours, immerse them in 0.01mol / L PDDA aqueous solution for 10-15 minutes, take them out and wash them with ultra-clean water. ...

Embodiment 2

[0102] Example 2 Using biochips to detect abnormal sugar chain IgA nephritis

[0103] 1. Laying gold film

[0104] 1.1 Prepare gold sol by trisodium citrate reduction method: take 100mL of 0.01% chloroauric acid aqueous solution and heat to boil, add 0.75ml of 1% trisodium citrate aqueous solution while stirring, and the golden yellow chloroauric acid aqueous solution turns into Purplish red, continue to boil for 15 minutes, make up to the original volume with distilled water after cooling.

[0105] 1.2 Gold film laying:

[0106] 1.2.1 After pickling and cleaning the slides, put them in N-β-(aminoethyl)-γaminopropyl trivalent oxysilane (APTMS) / toluene solution and reflux for 12 hours, then wash them, take them out, and dry them with nitrogen , put in a clean and dry place for later use;

[0107] 1.2.2 After soaking the treated glass slide in the nano-gold solution for 12 hours, immerse it in 0.01mol / L PDDA aqueous solution for 15 minutes, take it out and wash it with ultra-...

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Abstract

The invention relates to the field of biological chips, in particular to a protein chip for detecting a specific glycoprotein, antibody or antigen by surface plasmon resonance technology and a surface plasmon resonance principle-based detection method. The chip is provided with a metal film on a substrate; a connection layer is fixed on the surface of the metal film; agglutinin or other antigen or antibody is fixed on the connection layer; and the connection layer is prepared by activating a self-assembled monomolecular layer consisting of long-chain mercaptoethanol of which one end is provided with an active group and the other end reacts with the metal film through mercapto. The chip diagnoses diseases by the specific binding of the agglutinin and the specific glycoprotein and the binding of the specific antigen and antibody and provides a new way for disease diagnosis. The method is simple and practical, has low cost, simplifies operation steps, shortens detection time and is a novel method suitable for popularization.

Description

technical field [0001] The invention relates to the field of biochips, in particular to a biochip using surface plasmon resonance technology to detect specific sugar chain proteins, antibodies or antigens and a detection method based on the principle of surface plasmon resonance. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon. When the polarized light parallel to the surface is totally reflected on the interface at an incident angle called the surface plasmon angle, the incident light is coupled into the surface plasmon. At this angle, the interface reflectance will be significantly reduced due to the surface plasmon resonance. SPR is very sensitive to the refractive index of the dielectric attached to the metal surface, which is an inherent characteristic of all materials. Therefore, any dielectric attached to the metal surface can be detected, and different dielectrics have different surface plasmon angles. For the same mat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/574G01N21/41
Inventor 刘睿强
Owner 上海睿昂基因科技股份有限公司
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