A kind of production method of live Bacillus subtilis preparation for feed
A technology of Bacillus subtilis and live bacteria preparation, applied in animal feed, animal feed, bacteria and other directions, can solve the problems of high cost, easy to be infected by miscellaneous bacteria, unfavorable for large-scale promotion and use, and achieve stable quality and inhibit miscellaneous bacteria. Reproduction, beneficial effects on animal growth
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[0016] The technical scheme of the present invention is described in further detail below:
[0017] A kind of production method of live Bacillus subtilis preparation for feed, described live bacteria preparation for feed is produced according to the following method:
[0018] (1) Activation and cultivation of Bacillus subtilis strains
[0019] Inoculate the freeze-dried Bacillus subtilis species (such as: CGMCC.NO.0321) into the broth culture solution, culture at 37°C for 24 hours, then transfer to the nutrient plate medium by streaking, and culture in the incubator at 37°C for 24 hours , observe the situation of the transparent circle, select a single colony in the transparent circle for pure culture, and obtain purely cultured Bacillus subtilis.
[0020] (2) Mutation and screening of Bacillus subtilis strains
[0021] Use the Bacillus subtilis obtained in the previous step to make a bacterial suspension, put the bacterial suspension in a petri dish, place it on an electrom...
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