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Method for preparing antibacterial nanofibre composite membrane by utilizing electrostatic spinning and application thereof

A nanofiber, electrospinning technology, applied in fiber processing, fiber chemical characteristics, filament/thread forming, etc., can solve the research on active ingredients of tea polyphenols and complexation reactions, prone to redox reactions, affecting applications, etc. problems, to achieve good toughness and biocompatibility, mild conditions, and easy operation.

Inactive Publication Date: 2011-02-02
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above studies did not conduct in-depth research on the active ingredients and complexation reactions in tea polyphenols. At the same time, the complexes formed by catechins and copper ions are unstable in solution and prone to redox reactions, which affects their use in the field of biomedicine. Applications

Method used

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  • Method for preparing antibacterial nanofibre composite membrane by utilizing electrostatic spinning and application thereof
  • Method for preparing antibacterial nanofibre composite membrane by utilizing electrostatic spinning and application thereof
  • Method for preparing antibacterial nanofibre composite membrane by utilizing electrostatic spinning and application thereof

Examples

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Effect test

Embodiment 1

[0037] (1) Take 3ml of 5×10 -3 mol / l EGCG solution in the cuvette, then add 5μl 0.01 mol / l CuSO with a micropipette 4 Solution, with UV-Visible full-band spectrophotometer full-band detection, the results are as follows figure 1 shown.

[0038] (2) Weigh PVA (polyvinyl alcohol) and dissolve it in distilled water, heat to 95°C to fully dissolve, then add the solution in step (1), the final concentration of PVA is 8% (w / v, g / ml) , magnetically stirred at room temperature for 2 h for later use. Inhale the spinning solution into a 20ml syringe, install a needle with a diameter of 0.6mm, adjust the distance from the needle to the receiver to 6cm, adjust the voltage at 12kv, control the flow rate of the spinning solution at 2ml / h, spin for 2h, and vacuum dry at 80°C for 6h , to obtain EGCG_Cu (II) nanocomposite film.

Embodiment 2

[0040] (1) Take 3ml 5×10 -3 mol / l EGCG solution in the cuvette, then add 10μl 0.01 mol / l CuSO with a micropipette 4 Solution, with UV-Visible full-band spectrophotometer full-band detection, the results are as follows figure 2 shown.

[0041] (2) Weigh PVA (polyvinyl alcohol) and dissolve it in distilled water, heat to 95°C to fully dissolve, then add the solution in step (1), the final concentration of PVA is 8% (w / v, g / ml) , magnetically stirred at room temperature for 2 h for later use. Inhale the spinning solution into a 20ml syringe, install a needle with a diameter of 0.6mm, adjust the distance from the needle to the receiver to 6cm, adjust the voltage at 12kv, control the flow rate of the spinning solution at 2ml / h, spin for 2h, and vacuum dry at 80°C for 6h , to obtain EGCG_Cu (II) nanocomposite film.

Embodiment 3

[0043] (1) Take 3ml 5×10 -3 mol / l EGCG solution in the cuvette, then add 15μl 0.01 mol / l CuSO with a micropipette 4 Solution, with UV-Visible full-band spectrophotometer full-band detection, the results are as follows image 3 shown.

[0044] (2 The details are as follows: Weigh PVA (polyvinyl alcohol) and dissolve it in distilled water, heat it to 95°C to fully dissolve it, then add the solution in step (1), the final concentration of PVA is 8% (w / v, g / ml), magnetically stirred at room temperature for 2 hours for later use. Inhale the spinning solution into a 20ml syringe, install a needle with a diameter of 0.6mm, adjust the distance from the needle to the receiver to 6cm, adjust the voltage at 12kv, and control the flow rate of the spinning solution at 2ml / h. Spinning for 2 hours, vacuum drying at 80°C for 6 hours to obtain EGCG_Cu(II) nanocomposite membrane.

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Abstract

The invention belongs to the fields of nano material preparation technique and biological material, in particular to a method for preparing an antibacterial EGCG (Epigallocatechin Gallate)_Cu(II) / PVA (Polyvinyl Alcohol) nanofibre composite membrane by utilizing electrostatic spinning and an application thereof. The method of the invention comprises the following steps of weighing copper sulfate and EGCG according to a ratio; dissolving in distilled water; regulating the pH value to prepare an EGCG_Cu (II) complex; mixing the EGCG_Cu (II) complex and PVA according to a ratio; and generating the EGCG_Cu (II) / PVA nanofibre composite membrane by utilizing an electrostatic spinning technique. The invention has the advantage that the sterilizing rate on escherichia coli can reach 99%. The nanofibre composite membrane has the advantages of good toughness and biocompatibility, and wide application prospects in the field of the biological materials.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation technology and biological materials, and in particular relates to a method for preparing an antibacterial EGCG_Cu(II) / PVA nanofiber composite membrane by electrospinning and its application. Background technique [0002] Catechin is the main component of tea polyphenols, a phenolic substance rich in polyhydrocarbon groups in tea. EGCG (epigallocatechin gallate) is the most abundant catechin in tea, and has antibacterial and antibacterial effects. Due to the special structure of EGCG with multiple phenolic hydroxyl groups and strong acid-base buffering capacity, it is very easy to adsorb and complex with metal ions to form complexes when it meets metal ions. Japanese scholar Kimura et al. found that the bactericidal effect of tea polyphenols was significantly increased in the presence of copper ions. [0003] Electrospinning is a nanomaterial preparation technology with broad application pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D01F6/50D01D5/00
Inventor 李平孙立明
Owner TONGJI UNIV
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