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Saccharomyces cerevisiae strain and application thereof

A technology of Saccharomyces cerevisiae and alcohol, which is applied in fermentation, fungi, microorganisms, etc., and can solve problems such as difficulty in meeting higher requirements

Active Publication Date: 2011-02-02
广西鼎乐生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saccharomyces cerevisiae, which is widely used in the production of domestic alcohol industry, is the high-temperature-resistant active dry yeast (Ange Yeast, referred to as AQ) produced by Hubei Angel Yeast Co., Ltd. Although the fermentation characteristics are good, it is difficult to meet the higher requirements of the industry.

Method used

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  • Saccharomyces cerevisiae strain and application thereof
  • Saccharomyces cerevisiae strain and application thereof
  • Saccharomyces cerevisiae strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, the acquisition of yeast strain ZM1-5

[0041] 1. The acquisition of yeast strain ZM1-5

[0042] 1. Sample collection

[0043] Sample: Orchard soil. Collection location: rural area of ​​Qinzhou City, Guangxi Zhuang Autonomous Region.

[0044] 2. Isolation of yeast strains

[0045] Separation by dilution plate method: Weigh 1g of soil into a 100mL Erlenmeyer flask, add 10mL of sterilized distilled water, place on a constant temperature shaker at 28°C with a rotation speed of 180rpm for 2 hours, take 1mL of the suspension and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Dilution: Take 100 μL of each dilution gradient to coat the YPD plate, place it in a constant temperature incubator at 32°C for 2 days, and then pick out a single colony.

[0046] 3. Screening of yeast strains

[0047] The specific screening steps of yeast are as follows:

[0048] ①Observe the shape of the colony grown on the plate, and examine its cell shape under the microscope, pu...

Embodiment 2

[0061] Embodiment 2, the comparison of the optimal growth pH value of Saccharomyces cerevisiae ZM1-5 and Angelica AQ

[0062] Saccharomyces cerevisiae ZM1-5 and Angelica AQ were treated under exactly the same conditions to determine the optimum pH value for growth.

[0063] 1. Cultivate the yeast (Saccharomyces cerevisiae ZM1-5 or Angelica AQ) overnight, and detect the OD of the bacterial liquid 600 value.

[0064] 2. Press OD 600 =0.01 final concentration, insert the bacterial solution into YPD liquid medium with different pH values ​​(3, 4, 5, 6, 7, 8 or 9), cultivate at 28°C and 180rpm for 24 hours, and use a spectrophotometer at a wavelength of 600nm Determination of optical density value (OD 600 value).

[0065] Three replicates were set up, and the results were averaged. See the comparison between ZM1-5 and AQ growth for 24 hours. Figure 9 . The optimum growth pH of Saccharomyces cerevisiae ZM1-5 and Angelica AQ was 4-5.

Embodiment 3

[0066] Embodiment 3, the comparison of the optimal growth temperature of Saccharomyces cerevisiae ZM1-5 and Angelia yeast AQ

[0067] Saccharomyces cerevisiae ZM1-5 and Angelica AQ were treated under exactly the same conditions to determine the optimum growth temperature.

[0068] 1. Cultivate the yeast (Saccharomyces cerevisiae ZM1-5 or Angelica AQ) overnight, and detect the OD of the bacterial liquid 600 value.

[0069] 2. Press OD 600 = 0.01 final concentration Put the bacterial liquid into YPD liquid medium (pH4.5), culture at different temperatures (28°C, 32°C, 35°C, 37°C, 40°C or 42°C) and 180rpm for 20 hours, and detect the bacterial liquid OD 600 value.

[0070] Three replicates were set up, and the results were averaged. See the comparison of the bacterial mass of ZM1-5 and AQ grown for 20 hours under different temperature conditions Figure 10 . The optimal growth temperature of Saccharomyces cerevisiae ZM1-5 and Angelica AQ was 32°C, and the growth of Sacchar...

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Abstract

The invention discloses a saccharomyces cerevisiae strain and application thereof. The invention provides the saccharomyces cerevisiae ZM1-5 CGMCC No.3761. The saccharomyces cerevisiae ZM1-5 can be used for producing alcohol. The saccharomyces cerevisiae ZM1-5 has many advantages of quick growth, high alcohol yield, high temperature resistance, high sugar resistance, high-concentration ethanol resistance, acid resistance and the like, can be applied in a thick mash fermentation alcohol producing process (synchronous saccharification and fermentation) using cassava meal, molasses, sugar cane juice and the like as raw materials, solves the problems that the saccharomyces cerevisiae in the industrial production cannot tolerate high osmotic pressure and high-concentration ethanol and the efficiency at the later fermentation stage is low and the like, and reduces the cost of alcohol industrial production. The saccharomyces cerevisiae ZM1-5 can be used in the synchronous saccharification and fermentation alcohol producing process, and is expected to solve the problems of high substrate concentration (high osmotic pressure) in thick mash fermentation, high temperature in a fermentation tank, high-concentration alcohol inhibition for saccharomyces cerevisiae at the later fermentation stage and the like.

Description

technical field [0001] The invention relates to a strain of Saccharomyces cerevisiae and its application. Background technique [0002] Yeast is a general term for a class of unicellular fungi, not a unit of phylogenetic classification. Yeast is the earliest microorganism used in the history of human civilization. There are currently more than 1,000 species of yeast known. According to the ability of yeast to produce spores (ascospores and basidiospores), yeast can be divided into three categories: strains that form spores belong to ascomycetes or basidiomycetes, and strains that do not form spores but mainly through Those that reproduce by budding are called incomplete fungi, or "pseudoyeasts" (Kreger-van R, Groningen N Y. The yeasts: a taxonomic study. Third revised and enlarged edition [M]. The Netherlands: Elsevier science publishers B.V, 1984.). It is known that most yeasts are classified into the phylum Ascomycota. [0003] Yeasts are widely distributed in nature a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12R1/865C12N1/18C12P7/06
CPCY02E50/17Y02E50/10
Inventor 冯家勋罗雪梅
Owner 广西鼎乐生物科技有限公司
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