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Trichoderma viride fermented extract improves the method of alcoholic fermentation yield

A technology of Trichoderma viride and alcohol fermentation, applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of limited product use effect and high production cost, achieve obvious use effect, reduce production cost and price cheap effect

Active Publication Date: 2018-10-02
浙江全柱科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also fermentation synergists such as Yenqi fermentation synergists and brewing fermentation synergists that can improve the efficiency of alcohol fermentation, but due to high production costs and limited product use effects, few of them have been widely used in actual production

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  • Trichoderma viride fermented extract improves the method of alcoholic fermentation yield
  • Trichoderma viride fermented extract improves the method of alcoholic fermentation yield
  • Trichoderma viride fermented extract improves the method of alcoholic fermentation yield

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Embodiment Construction

[0018] The present invention will be further described below in conjunction with drawings and embodiments.

[0019] As shown in the figure: the method that a kind of Trichoderma viride fermented extract of the present invention improves alcohol fermentation output comprises the following steps at least:

[0020] (1) Solid-state fermentation with straw as raw material

[0021] Add straw powder into the fermentation tank, then add water and ammonium nitrate, and stir well, then insert Trichoderma viride CGMCC3.6619, so that the bacteria and straw powder can fully contact; continue to cultivate in a constant temperature room at 28°C for one month, after fermentation Dried to obtain the fermentation substrate for subsequent use;

[0022] (2) Extraction and purification of extract alcohol

[0023] Use 3.5% NaOH solution to extract the dried fermentation substrate obtained in step (1), filter it in a constant temperature water bath at 60°C for 1 hour, and then concentrate the filt...

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Abstract

The present invention discloses a method for increasing the alcohol fermentation yield through a Trichoderma viride fermentation extract. The method at least comprises: (1) adopting straws as a raw material to carry out solid state fermentation, wherein straw powder is added to a fermentation barrel, water and ammonium nitrate are added, complete stirring is performed, Trichoderma viride CGMCC3.6619 is inoculated to make the strain and the straw powder completely contact, continuous culturing is performed for one month, and drying is performed after completing the fermentation to obtain a fermentation matrix for spare; (2) carrying out extraction and purification on the extract alcohol fermentation auxiliary agent, wherein the fermentation matrix is soaked with a NaOH solution, constant temperature treatment and filtration are performed, the filtrate is concentrated to prepare a raw liquid, the pH value of the raw liquid is adjusted to 2.0, standing, centrifugation and precipitate removing are performed, and the supernatant is subjected to spray drying to a product alcohol fermentation auxiliary agent; and (3) carrying out alcohol fermentation, starch is taken and added to clear water, stirring is performed to make the starch be uniformly dispersed in the water, liquid high-temperature amylase and calcium chloride are added, the pH value is adjusted, liquid glucoamylase is added, cooling is performed after completing the saccharification, activated yeast and the alcohol fermentation auxiliary agent are added, and fermentation is performed in a constant temperature chamber.

Description

technical field [0001] The invention belongs to a method for improving alcohol fermentation yield by preparing a mixture with biological activity through microbial strains. Background technique [0002] Fuel alcohol (ethanol) is one of the three new energy sources in the 21st century, and high-concentration alcohol fermentation is an inevitable trend and one of the technical bottlenecks in the development of the industry. Using reasonable process conditions to improve the tolerance of yeast to high-concentration alcohol can improve yeast production. Alcohol capacity has received a lot of attention. There are various factors affecting the fermentation of high-concentration alcohol, and for these limiting factors, people's countermeasures are also tried in every possible way. On the basis of groundbreaking research on strain selection, equipment improvement, and medium optimization, some people focused on the efficient use of existing equipment and processes, and adopted a co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12R1/885C12R1/865
CPCC12P7/10C12P19/14Y02E50/10
Inventor 辅琳瑜李加友安鹏邱浩峰刘丽何鑫奕
Owner 浙江全柱科技有限公司
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