Use of bdellovibrio bacteriovorus bdelloplast as bacteria remover in fruit cleaning

A technology of Bdellovibrio and antibacterial agent, which is applied in the field of application of Bdellovibrio brucoplasma as an antibacterial agent in cleaning fruit, which can solve the problem of ineffective elimination of microorganisms, pollution of water body and atmosphere, and inability to kill pathogenic bacteria. Bacteria and other problems, to avoid fruit food poisoning incidents, strong environmental tolerance, and eliminate the probability of food poisoning

Inactive Publication Date: 2011-02-02
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the industrial cleaning process of fruits mainly adopts the combination of physical cleaning and chemical fungicides. First, different cleaning equipment is selected according to different types of fruits, such as DT5A1 cleaning machine, blower cleaning machine, XGJ-2 cleaning The main principle of the machine is the friction and interaction between the fruit and the washing water. At the same time, it is assisted by the brush roller to clean the dirt on the surface of the fruit; secondly, choose a suitable cleaning agent. There are many types of cleaning agents on the market. , decontamination, and bactericidal function, but the traditional cleaning method is good for removing the soil in fruits and vegetables, but the effect of eliminating microorganisms is not obvious
Most of the currently used fruit fungicides are chlorine-based fungicides, but these can not kill pathogenic bacteria such as Escherichia coli O157:H7 strains, Salmonella, Shigella, Listeria monocytogenes, golden yellow Staphylococcus, etc., but also react with certain ingredients in food to form chloride, which is harmful to the human body. The cleaned wastewater contains residual chlorine, which reacts with organic matter in water to form chloroform, which not only pollutes water bodies and the atmosphere, but also carcinogenic to humans

Method used

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  • Use of bdellovibrio bacteriovorus bdelloplast as bacteria remover in fruit cleaning

Examples

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Effect test

Embodiment 1

[0035] Elimination of Common Pathogenic Bacteria on Apple Surface

[0036] (1) Preparation of Bdellovibrio Bdeloidoplasts Eliminating Pathogenic Bacteria on Apple Surface

[0037] The Bdellovibrio BDM01 is obtained by ultraviolet mutagenesis of Bdellovibrio BDJ01, and its morphology is observed under an electron microscope after negative staining: Bdellovibrio BDM01 is single-celled, arc-shaped, 1.7×1.0 μm in size, terminal Flagella, the length of the flagella is 3.5 μm; it can form a transparent circular plaque with a diameter of 3-4 mm when it is cultured at 28 ° C for three days with a double-layer plate method.

[0038] Preparation of Bdellovibrio Brucoplasma: Inoculate 10mL×10 10 cfu / mL Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172), 250rpm, 30°C shaker culture for 24 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. The precipita...

Embodiment 2

[0050] Elimination of common pathogenic bacteria on the surface of muskmelon

[0051] Step (1), (2) and (3) are all with embodiment 1

[0052] Test results such as image 3 , as shown in 4:

[0053] The total number of bacterial colonies and the residual amount of pathogenic bacteria in the experimental group after using the Bdellovibrio bacteroid plastid as a fungicide were far lower than those simply washed with tap water, and the higher the concentration of Bdellovibrio, the higher the total number of bacterial colonies and The effect of pathogenic bacteria is better, the minimum concentration is 10 2 The elimination rate of pfu / mL for pathogenic bacteria has reached 94%. However, Bdellovibrio eliminates pathogenic bacteria by cracking on the surface of the fruit, which will not affect the quality of the fruit, nor will it cause the residue of the fungicide in the fruit. Fast, efficient and safe degerming makes Bdellovibrio great potential in eliminating pathogenic bact...

Embodiment 3

[0055] Comparison of sterilization effects between new bactericides and traditional bactericides

[0056] Step (1) (2) is all the same as embodiment 1

[0057] Step (3) the elimination test of different bactericides to pathogenic bacteria

[0058] Plums were selected as the effect fruit and divided into experimental group and control group.

[0059] All the plums in the control group and the experimental group were first sterilized by 75% alcohol, then soaked in the mixed bacterial solution prepared in step (2) for 1 min, taken out, drained, and then sterilized with different bactericidal agents.

[0060] Control group: three plums were taken as three parallel samples, and the plums were soaked in tap water;

[0061] Experimental group: divided into four groups, each group took three plums, as three parallel samples

[0062] (1) Soak the plums in a concentration of 10 2 pfu / mL Bdellovibrio bdelloplast cleaning water;

[0063] (2) plums are soaked in the cleaning water tha...

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Abstract

The invention discloses the use of bdellovibrio bacteriovorus bdelloplast as a bacteria remover in fruit cleaning. The use is characterized in that: bdellovibrio bacteriovorus bdelloplast liquid is added into cleaning water as the bacteria remover for cleaning the fruits; or the bdellovibrio bacteriovorus bdelloplast liquid is sprayed into processing environment for cleaning the processing environment; and the bdellovibrio bacteriovorus is bdellovibrio bacteriovorus (Bdellovibrio sp.)BDM01, and was collected in the China Center for Type Culture Collection on April 28th, 2008 with the collection number of CCTCC No.M208066. The bdellovibrio bacteriovorus bdelloplast has strong effect of eliminating common disease germs on the fruit surface, the disease germ eliminating rate of the bdellovibrio bacteriovorus bdelloplast reaches over 93 percent, and the bdelloplast has the advantages that: the preparation and collection of the bdelloplast are more convenient; the environmental resistance of the bdelloplast is higher; the sterilizing effect of the bdelloplast lasts for a longer time; and the like.

Description

technical field [0001] The invention relates to the application of the Bdellovibrio bdeloidoplast, in particular to the application of eliminating the total number of bacterial colonies and pathogenic bacteria carried in the fruit surface and its processing process. Background technique [0002] Food safety has become a common concern all over the world. Various materials and statistical data at home and abroad show that microorganisms are the chief culprit of foodborne diseases. As "raw food therapy" is gradually regarded as a healthy fashion, large-scale food poisoning outbreaks caused by raw fruit consumption are frequent. Pollution. Therefore, it is very important to keep fruits and vegetables fresh by removing or killing pathogenic microorganisms in fruits and vegetables through cleaning and sterilization technology to achieve the purpose of preventing and controlling infectious diseases caused by fruits and vegetables. [0003] At present, the industrial cleaning pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015C12N1/20C12R1/01A23L5/20
Inventor 蔡俊鹏陈丽芸
Owner SOUTH CHINA UNIV OF TECH
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