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Bdellovibrio bacteriovorus preparation and fermentation method and application thereof

A fermentation method and leech vibrio technology, applied in the field of fermentation, can solve the problems of low leech vibrio preparation content and low lysis activity, and achieve the effects of shortening the fermentation period, high activity, and increasing the scope of use

Inactive Publication Date: 2011-01-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the problems of low content and low cracking activity of Bdellovibrio preparations prepared by existing methods, the primary purpose of the present invention is to provide a fermentation method for Bdellovibrio preparations

Method used

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  • Bdellovibrio bacteriovorus preparation and fermentation method and application thereof
  • Bdellovibrio bacteriovorus preparation and fermentation method and application thereof
  • Bdellovibrio bacteriovorus preparation and fermentation method and application thereof

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Embodiment 1

[0050] Bacillus subtilis (strain number: GIM 1.136, sourced from the Guangdong Provincial Culture Collection Center for Microorganisms) and Escherichia coli (strain number: GIM 1.137, sourced from the Guangdong Provincial Culture Collection Center for Microorganisms) were used as host bacteria, respectively. Prepare Bdellovibrio preparation A and Bdellovibrio preparation B, the specific process is as follows:

[0051] (1) Preparation of Bacillus subtilis suspension

[0052] Bacillus subtilis was inoculated in nutrient broth medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2), and cultured in a shaker at 33°C for 18h, so that it was in the logarithmic growth phase. Centrifuge the culture solution at 5000rpm for 25min at 4°C, retain the precipitate and discard the supernatant, add sodium potassium phosphate buffer solution (concentration: 0.2mol / L, pH 7.6) to the precipitate to obtain a concentration of 10 19 cfu / mL of the Bacillus subtilis suspension, ...

Embodiment 2

[0061] Bdellovibrio preparation A and Bdellovibrio preparation B were prepared with Bacillus natto (strain number: CGMCC 1.1086, derived from the General Microbiology Center of China Microbiological Culture Collection Management Committee) and Escherichia coli as host bacteria. The specific process is as follows:

[0062] (1) Preparation of Bacillus natto suspension

[0063] Inoculate Bacillus natto in nutrient broth medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH 7.4±0.2), and culture it in a shaker at 35°C for 15h, so that it is in the logarithmic growth phase , the culture solution was centrifuged at 6000rpm for 25min at 4°C, the precipitate was retained and the supernatant was discarded, and sodium phosphate buffer (concentration: 0.1mol / L, pH 7.4) was added to the precipitate to obtain a concentration of 10 18 The suspension of Bacillus natto of cfu / mL is then placed in a refrigerator at 2°C for subsequent use;

[0064] The same method as above, the p...

Embodiment 3

[0072] Bdellovibrio preparation A and Bdellovibrio preparation B were prepared with Bifidobacterium bifidum (strain number: GIM 1.169, derived from Guangdong Microbial Culture Collection Center) and Salmonella typhimurium as host bacteria respectively. The specific process is as follows:

[0073] (1) Preparation of Bifidobacterium bifidum suspension

[0074] Inoculate Bifidobacterium bifidum in PTYG medium, culture in a shaker at 37°C for 24 hours to make it in the logarithmic growth phase, centrifuge the culture solution at 8°C at 7000rpm for 20min, retain the precipitate and discard the supernatant , add potassium phosphate buffer solution (concentration is 0.3mol / L, pH 7.5) to precipitation, obtain concentration is 10 22 cfu / mL Bifidobacterium bifidum suspension, and then placed in a refrigerator at 8°C for future use;

[0075] In the same way as above, use the nutrient broth medium to prepare a concentration of 10 22 The Salmonella typhimurium suspension of cfu / mL is sto...

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Abstract

The invention discloses a bdellovibrio bacteriovorus preparation and a fermentation method and application thereof. The fermentation method comprises the following steps of: (1) preparation of host bacteria suspension; (2) preparation of bdellovibrio bacteriovorus swimming body concentrated solution; and (3) preparation of the bdellovibrio bacteriovorus preparation. Host bacteria adopted in the fermentation method are Gram positive bacteria which are beneficial or harmless to the environment, the method can keep and even improve the capability of bdellovibrio bacteriovorus for cracking certain Gram positive bacteria, and the bdellovibrio bacteriovorus preparation prepared by using the method has certain improvement on the cracking capability of pathogenic bacteria; and the prepared bdellovibrio bacteriovorus preparation can be directly used without more fermentation post treatment, so the use range of the preparation is greatly increased. The method relatively shortens the fermentation period at the same time of obtaining high-concentration bdellovibrio bacteriovorus bacterial solution so as to effectively solve the problems of over-high energy consumption and over-high productioncost caused by overlong fermentation time; and the obtained bdellovibrio bacteriovorus preparation has low cost and high activity.

Description

technical field [0001] The invention relates to the technical field of fermentation, in particular to a Bdellovibrio preparation and a fermentation method and application thereof. Background technique [0002] At present, the main means of controlling and eliminating pathogenic bacteria are still various physical and / or chemical methods, including the use of various antibiotics. Both physical and / or chemical methods have obvious disadvantages. First, the excessive abuse of antibiotics will lead to some side effects, such as drug resistance of pathogenic bacteria and suppression of beneficial flora. Secondly, although the method of acid-base treatment can achieve a certain sterilization / bactericidal effect, after the harmful bacteria form a biofilm, the effect of these methods is extremely unsatisfactory. Finally, the elimination of various pathogenic bacteria by physical methods can only be applied to a certain link in the related field, and it is difficult to extend to all...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61P31/00C12R1/01
CPCY02A50/30
Inventor 蔡俊鹏陈小红
Owner SOUTH CHINA UNIV OF TECH
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