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Preparation method of stevioside

A technology of stevia and raw materials, applied in the field of preparation of stevia, can solve the problems of large solvent consumption, residual organic solvent and high cost, and achieve the effects of reducing production cost, reducing regeneration cost and high purity

Active Publication Date: 2011-01-19
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for extracting stevioside from Stevia rebaudiana leaves mainly include organic solvent extraction and water extraction. Among them, the organic solvent extraction method consumes a lot of solvent, has high cost, and is not practical. Less residual organic solvents
Hot water extraction method is a widely used extraction method at present, but the purity of stevioside products extracted by the existing hot water extraction method is not ideal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take stevia leaves as raw material, add 10 times the weight of the raw material for leaching at room temperature for 5 hours, the extract is first filtered through an alumina ceramic membrane with a pore size of 0.25 μm, and the filtrate is filtered with an ultrafiltration membrane with a molecular weight cut-off of 300-800 Daltons. Ultrafiltration (working pressure is 0.1MPa), ultrafiltration permeate adds the ferrous sulfate of raw material weight 4% and stirs for 10min with lime milk to adjust the pH value of solution to 9, centrifuge, supernatant passes 717# anion resin, flows out The liquid is then passed through 732# cation resin, and the effluent of the cation resin is then decolorized by a D941 macroporous decolorizing resin column.

[0019] It was determined that the purity of the stevioside prepared above was 85.2%, and the content of RA was 48.5%.

Embodiment 2

[0021] Take stevia leaves as raw material, add 10 times the weight of the raw material for leaching at room temperature for 2 hours, filter, and then enter the filter residue into 8 times the weight of the raw material for leaching at room temperature for 2 hours, filter, and combine the extracts. The filtrate is filtered with an ultrafiltration membrane with a molecular weight cut-off of 300 to 900 Daltons (working pressure is 0.2MPa), and the ultrafiltration permeate is added with 7% ferrous sulfate by weight of the raw material and stirred for 5 minutes with lime. The pH value of the solution is adjusted to 8.5, centrifuged, the supernatant is passed through 717# anion resin, the effluent is passed through 732# cation resin, the effluent of the cation resin is then decolorized by the activated carbon column, the effluent of the activated carbon column is collected, suction filtration, The filtrate is concentrated and spray-dried to obtain stevia.

[0022] It was determined ...

Embodiment 3

[0024] Take stevia leaves as raw material, add 12 times the weight of the raw material, 50 ° C water for 4 hours, the extract is first filtered through an alumina ceramic membrane with a pore size of 0.5 μm, and the filtrate is filtered with a molecular weight cut-off of 300-800 Daltons. Ultrafiltration membrane ultrafiltration (working pressure is 0.3MPa), the ultrafiltration permeate is added with 2% ferrous sulfate by weight of the raw material, stirred for 8min, and the pH value of the solution is adjusted to 9.5 with lime milk, centrifuged, and the supernatant is passed through 717# anion Resin, the effluent is passed through 732# cation resin, the effluent of the cation resin is then decolorized by the activated carbon column, the effluent of the activated carbon column is collected, suction filtered, the filtrate is concentrated, and spray-dried to obtain stevia.

[0025] It was determined that the purity of the stevioside prepared above was 85.0%, and the content of RA ...

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Abstract

The invention discloses a preparation method of stevioside, which comprises the steps of: adding water to stevia leaf, which is used as raw material, for extraction once or twice, mixing extracting solutions, filtering the extracting solution by a ceramic membrane and then ultra-filtering the filtering solution by an ultrafiltration membrane, adding ferrous sulfate to ultrafiltration permeating solution and then mixing for 5 to 10 minutes, using lime milk for adjusting pH of the mixture to 8 to 9.5, centrifuging the mixture, bringing supernatant to an anion resin column, a cation resin column and a decolorizing resin column in sequence, collecting the effluent from the decolorizing resin column, pump-filtering the effluent, and concentrating the filtrate and drying to obtain the stevioside, wherein the ceramic membrane is an alumina ceramic membrane with the pore diameter ranging from 0.25 to 1 micron; the ultrafiltration membrane has the cut-off molecular weight ranging from 300 to 900 Daltons and the working pressing ranging from 0.1 to 0.3 MPa. Compared with the prior art, the preparation method according to the invention guarantees the purity of the obtained stevioside product while lowering the production cost, in addition, the content of effective ingredients in the stevioside product can reach more than 80%, with the content of RA up to more than 45%.

Description

technical field [0001] The invention relates to a preparation method of stevioside, and belongs to the technical field of extraction of active ingredients in plants. Background technique [0002] Stevia is a perennial herb belonging to the Asteraceae family, native to the subtropical regions of South America. Stevia, also known as stevioside, is a mixture of diterpene glycosides containing 8 components extracted from the leaves of Stevia. According to the division of natural phytochemistry, it belongs to the glycosides of tetracyclic diterpenes. The characteristics of high temperature, low heat, safety and non-toxicity are gradually favored by people. Among the 8 known glycosides of stevioside, the content, taste and sweetness of various components are different, among which stevioside (Stevioside), rebaudioside A (RA), rebaudioside C (RC) The content is relatively high, accounting for more than 90%. RA has the highest sweetness, equivalent to 200 to 350 times that of suc...

Claims

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Application Information

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IPC IPC(8): A23L1/236A23L27/30
Inventor 孙步祥梁远盛宋云飞李送军罗菲菲汤凌志
Owner GUILIN NATURAL INGREDIENTS CORP
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