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Fast propagation technology for wild buckwheat rhizome

A fast technology of buckwheat notoginseng, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., to achieve the effects of less floor space, short production cycle, and labor saving

Inactive Publication Date: 2011-01-19
石国荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there have been no reports on the success of buckwheat Panax notoginseng tissue culture rapid propagation at home and abroad

Method used

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  • Fast propagation technology for wild buckwheat rhizome
  • Fast propagation technology for wild buckwheat rhizome
  • Fast propagation technology for wild buckwheat rhizome

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Fresh buds, stems and other young tissues of buckwheat collected from Huaihua, Hunan, washed with tap water, soaked in 70% alcohol for 30s, rinsed twice with sterile water, and disinfected with 0.1% mercury 100ml for 10 minutes, and finally Rinse three times with sterile water. Connect the sterilized medium MS+2,4-D2mg / L+6-BA0.5mg / L (see figure 1 ). Cultivate for 4 to 5 days under dark conditions, then transfer to 40W single tube fluorescent lamp for culture, light intensity is 1000LX, temperature is maintained at 25±2℃, cultured for 15 days, a lot of green tissue appears around the explant (see figure 2 ), the success rate of inducing callus is over 98%; then transferred to MS+2,4-D2mg / L +6-BA0.5mg / L subculture medium for subculture for one month, and a large amount of callus is obtained (see image 3 ) And then transferred to MS+KT1.5mg / L+6-BA1.5mg / L differentiation medium for differentiation culture, a large number of seedlings can grow in 15 days (see Figure 4 ); T...

Embodiment 2

[0034] Fresh buds, stems and other young tissues of buckwheat collected from Huaihua, Hunan, washed with tap water, soaked in 70% alcohol for 30s, rinsed twice with sterile water, and disinfected with 0.1% mercury 100ml for 10 minutes, and finally Rinse three times with sterile water. Connect the sterilized medium Ms+KT1.5mg / L+6-BA1.5mg / L. When the light intensity is 2000LX, the temperature is maintained at 25±2℃, and the culture is 15 days, it can be seen that there are about 5 new buds on each explant. Take out the explants with buds under aseptic conditions, separate each bud with a scalpel, and place them in a culture container equipped with the above continuous medium. Within 10-20 days, The bud can regenerate several new buds. The buckwheat Panax notoginseng plants that need rooting are transferred to MS hormone-free medium for rooting culture. Rooting takes place in about 12 days, and the rooting rate reaches 100%; open the lid of the culture flask and cultivate for t...

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Abstract

The invention provides a fast propagation technology for wild buckwheat rhizome. The method comprises the following steps: culturing tender tissues such as fresh tender shoots and stems of the wild buckwheat rhizome on an MS culture medium; inoculating the tender tissues on the culture medium consisting of 2 mg / L of (MS+2,4-D) and 0.5 mg / L of (6-BA), wherein the success rate of callus induction is over 98%; transferring to a differential medium consisting of 1.5 mg / L of (MS+ KT) and 1.5 mg / L (6-BA) for differential culture, wherein a large number of seedlings appear after 15 days; transferring about 1.5 to 3.5-cm seedlings to a hormone-free MS rooting medium, wherein roots appear after about 12 days and the rooting rate reaches 100%; opening the cover of the culture bottle to culture for three days, taking out the tissue culture seedlings of the wild buckwheat rhizome, cleaning the rooting medium, and transplanting the seedlings to a greenhouse and growing on special nutrient soil for tissue culture, wherein the survival rate reaches 98%; and after three months, transplanting the seedlings to fields for large-scale planting. The method of the invention has the advantages of high stability, simple operation, fast propagation, low production cost, reached industrial level and the like.

Description

Technical field [0001] The invention relates to the field of biotechnology, in particular to a tissue culture rapid propagation method of buckwheat notoginseng. Background technique [0002] Plants are a natural treasure house of medicines. People have a long history of using medicinal plants. About 75% of the world's population use plants as a source of medicines for the treatment and prevention of diseases (Xing Jianmin, 2001). Mankind has discovered many drugs with high physiological activity from plants. Currently, drugs derived from plants account for more than 25% of the total drugs (Zheng Guangzhi, 1987). Traditional Chinese herbal medicine in my country has a history of thousands of years and is still widely used in my country and many countries and regions. However, because traditional Chinese herbal medicine acquisition methods are at the cost of collecting and consuming a large amount of wild plant resources, when the amount of collection and consumption exceeds the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 石国荣
Owner 石国荣
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