Breeding method of rice variety with stripe vein marked characters

Abstract

The invention relates to a breeding method of a rice variety with stripe vein marked characters, which is characterized by comprising the following steps: carrying out physicochemical mutation treatment by utilizing ion beams or gamma rays and ethylmethane sulfonate, inducing color variation of rice vein stripes, and screening rice vein mutants; selecting the mutant marked with vein stripes on blades for individually planting until the mutant is stably expressed; hybridizing and backcrossing the stripe vein mutation material with the conventional rice, analyzing the genetic rules of the character, hybridizing and backcrossing the stripe vein mutation material with the conventional rice and the parents of the hybrid rice, identifying the fertility, carrying out test-crossing and mating tests, and selecting two nuclear sterile lines marked with recessive stripe veins, a restoring line, three maintenance lines and a conventional rice material; and matching the two bred nuclear sterile lines, the bred restoring line and the bred maintenance lines, and verifying the validity to acquire hybrid rice parents, hybrid rice combination and conventional rice varieties marked with veins. The invention realizes the purposes of quickly, simply, conveniently, visually and accurately identifying the characteristics and the individualities of rice varieties and quickly and accurately identifying the purity of varieties in the seedling stage, and the invention increases new gene types of rice.

Description

technical field [0001] The invention relates to a method for breeding rice varieties marked with striped veins. Background technique [0002] Rice plays an important role in my country's grain production, and the production of rice seeds is the top priority of my country's food security. With the promulgation of the "Seed Law" and the rapid advancement of seed industrialization, the rice seed industry has received more and more attention from all walks of life. The continuous breeding of high-yield and high-quality new rice varieties has made a significant contribution to the increase in grain production and income in my country. However, incidents of counterfeit and shoddy seeds pitting farmers also continue to occur. On the one hand, the main reason is biological mixing (such as string powder) in the seed production process, parental mixing and mechanical mixing in the process of seed harvesting, processing, storage and transportation; Due to intellectual property rights,...

AI Technical Summary

Problems solved by technology

[0003] In the past, the traditional identification method was to identify a specific variety based on the morphological characteristics of the plant and the botanical classification method. For some varieties with no obvious differences between varieties, it was difficult to define and the effect was poor.

The DUS measurement technology currently used in the national variety protection is a very effective protection method, but due to the high content of identification technology, the units that can engage in technical identification are limited, the cost is high, and the application is limited.

[0004] There are known methods for marking rice leaf appearance traits, mainly using the difference in leaf color during the vegetative growth period of rice, such as using light green leaf traits, utilizing purple leaf traits, and using green-turning albino mutations to mark rice plants. Most of these leaf color marker traits affect the photosynthesis of rice, reduce the photosynthetic efficiency of rice, and have a certain impact on the yield traits of plants, so there are few varieties that can be applied in large-scale promotion

So far, there has been no report on the use of rice leaves with white, yellow, purple and other striped vein markers to breed rice parents and combinations. The vein marker traits are stably expressed during the whole growth period of rice plants, which can visually indicate the specificity of varieties. and authenticity, providing an intuitive and accurate method for the identification and protection of rice varieties. At the same time, the marker trait only exists in one vein in the leaf, and has no effect on the photosynthesis of rice leaves, and has no effect on rice yield traits. It will have an impact on the industrialization and application of rice varieties with this marker trait