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Genetic chip for detecting transgenic plants and products containing screening gene CaMV35S and application thereof

A technology for genetically modified plants and screening genes, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc. It can solve the problems of patent reports on gene chip detection technology that have not yet discovered the CaMV35S gene, and improve supervision. Level, good specificity, high sensitivity effect

Inactive Publication Date: 2011-01-05
山东鲁保科技开发有限公司
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] After searching the existing patents and other documents, no patent report on the gene chip detection technology of CaMV35S gene has been found

Method used

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  • Genetic chip for detecting transgenic plants and products containing screening gene CaMV35S and application thereof
  • Genetic chip for detecting transgenic plants and products containing screening gene CaMV35S and application thereof
  • Genetic chip for detecting transgenic plants and products containing screening gene CaMV35S and application thereof

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Embodiment 1

[0056] 1. Design of primers and probes

[0057] The primers were fluorescently labeled with Cy3-Amidite at the 5' end. The primers were synthesized by Shanghai Boya Biological Company, and the primers were diluted to 10 μmol / L for use.

[0058] Use Primer premier 5.0 and Oligo 6.0 software to design 40mer Oligo candidate probes based on the sequence of CaMV35S. The general principles of design are: (1) have relatively similar TM values, and the fluctuation range is 5°C; (2) GC content is 45% ~55%, otherwise the background of non-specific hybridization will be increased; (3) the repeated single bases shall not exceed 4 consecutively; (4) the length of the paired bases of the most stable secondary structure of the probe molecule shall not be greater than 4bp. The probe was modified with aminohexane at the 5' end, and the amino group and the probe sequence were connected with a spacer poly(dT15), so that the final probe length was 55 bp. All probes were synthesized in Shanghai ...

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Abstract

The invention discloses a genetic chip for detecting transgenic plants and products containing screening genes CaMV35S and application thereof. The genetic chip comprises a glass slide, a screening gene CaMV35S probe, a positive control (HEX), a hybrid positive control (PC), a positive quality-control probe (18S rRNA), a negative quality-control probe and a blank control, wherein the surface of the glass slide is modified in an aldehyde mode, the screening gene CaMV35S probe is coated on the glass slide in an arrayed way, and the positive control is fixed by nucleic acid. The screening gene CaMV35S probe has a sequence: NH2-(CH2)6-O-Poly (dT)15-CTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGA. The genetic chip for detecting the transgenic plants and the products containing the screening genes CaMV35S has better specificity and high sensitivity, provides a simple and reliable testing method for the detection and the analysis of the transgenic plants and the products containing the screening genes CaMV35S and can be used for detecting the transgenic plants and the products thereof.

Description

technical field [0001] The invention relates to a gene chip for detecting transgenic plants and products containing screening gene CaMV35S and an application thereof, belonging to the technical field of biochips. Background technique [0002] In recent years, genetically modified crops such as corn, soybean, cotton, rapeseed and tomato have been approved for planting and production in many countries, and some have been processed into food, feed or used as food additives. Since the ecological safety and food safety of genetically modified products have been controversial, more than 40 countries and regions have successively implemented genetically modified product labeling systems. [0003] The establishment of genetically modified labeling systems in various countries has put forward strict requirements on the sensitivity and accuracy of genetically modified detection technology, and various genetically modified detection technologies have also become research hotspots. Com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 路兴波武海斌孙红炜韩伟李凡
Owner 山东鲁保科技开发有限公司
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