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Extraction technology of gambogic acid

An extraction process, the technology of gambogic acid, which is applied in the field of medicine, can solve the problems of unfavorable industrial scale-up production, cumbersome operation steps, and low extraction yield, and achieve the effects of recyclable solvent, high purity, and fast extraction speed

Active Publication Date: 2010-12-22
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the operation steps are cumbersome, the operation process uses a variety of solvents, it is difficult to recover, the processing process is slow, and the extraction yield is low, which is not conducive to industrial scale-up production

Method used

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  • Extraction technology of gambogic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 A kind of extraction process of gambogic acid

[0018] Proceed as follows:

[0019] (1) Weigh Garcinia cambogia 6kg, pulverize into 50 mesh powder, add 12kg of silica gel (silica gel is pretreated in advance: add 8000mL methanol to silica gel to wash silica gel, to dryness. Wash silica gel with 8000mL of ethyl acetate again, to dryness ) uniformly mixed, filled into a chromatographic column with a diameter of 50 cm, and eluted with ethyl acetate. The eluent was initially dark brown, and the elution was stopped when the eluent turned pale yellow. Concentrate under reduced pressure at 40°C and recover the solvent to obtain a yellow primary extract of gambogic acid.

[0020] (2) Dissolve the primary extract of gambogic acid in pyridine (mass volume (g:ml) ratio 1:2), fully shake for 1 hour, add 1 volume of 50°C distilled water of the mixture, stir well, let stand, after cooling There was solid precipitation, suction filtration, and drying to obtain 1.89Kg of...

Embodiment 2

[0024] Embodiment 2 A kind of extraction process of gambogic acid

[0025] Proceed as follows:

[0026] (1) Weigh 6kg of Garcinia cambogia, pulverize it into 30 mesh powder, add 24kg of diatomaceous earth and mix evenly, fill it into a chromatographic column with a diameter of 80cm, elute with methanol, the eluent is initially dark brown, and the eluent is The elution was stopped when light yellow, the eluate was collected, the eluate was concentrated under reduced pressure at 40° C. and the solvent was recovered to obtain a yellow paste-like primary extract of gambogic acid.

[0027] (2) dissolving the first extract of gambogic acid in triethylamine (mass volume (g: ml) ratio 2: 1), fully shaken for 1 hour, add 4 times the volume of distilled water of the mixture and fully stir for 2 hours, after standing A solid was precipitated, which was filtered off with suction and dried to obtain 1.98Kg of crude gambogic acid triethylamine salt.

[0028] (3) the obtained gambogic acid...

Embodiment 3

[0031] Embodiment 3 A kind of extraction process of gambogic acid

[0032] Proceed as follows:

[0033] (1) take by weighing garcinia resin 6kg, pulverize into 60 mesh powder; Add 6kg quartz powder (quartz powder is pretreated in advance: add 8000mL methanol washing quartz powder in quartz powder, to dry. Wash with 8000mL ethyl acetate again Quartz powder, to dryness), evenly mixed, filled into a chromatographic column with a diameter of 50 cm, and eluted with an organic solvent (volume ratio of methanol and dichloromethane is 1:1), the eluent was initially dark brown, and the eluent was When light yellow, the elution was stopped, the eluate was collected, the eluate was concentrated under reduced pressure at 40° C. and the solvent was recovered to obtain a yellow primary extract of gambogic acid.

[0034] (2) dissolving the gambogic acid initial extract in pyridine (mass volume (g: ml) ratio 1: 3), fully shaken for 1 hour, add 2 times the volume of distilled water of the mix...

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Abstract

The invention relates to an extraction technology of gambogic acid, which comprises the following steps of firstly, crushing gamboge, uniformly mixing with silica, diatomite, quartz powder or polyacrylamide the quantity of which is 0.5-5 times as much as that of the crushed gamboge, installing a chromatography column, adding an organic solvent, washing, collecting eluent, concentrating the eluent, and recovering a solvent to obtain a gambogic acid primary extractive; secondly, dissolving the gambogic acid primary extractive into triethylamine or pyridine, fully oscillating to form a salt, adding distilled water, stirring, standing, pump filtering, and drying to obtain a crude gambogic acid salt product; thirdly, dissolving the obtained crude gambogic acid salt product into the solvent to prepare a saturated solution, crystallizing to obtain gambogic acid salt crystals, pump filtering, and drying to obtain gambogic acid salts; fourthly, extracting the gambogic acid salts with hydrochloric acid and diethyl ether, separating liquid, taking a diethyl ether layer, reducing the pressure, recovering a diethyl ether solution, and drying to finally obtain solid gambogic acid. The inventionhas the advantages of simple steps, recycled solvent, rapid extraction speed, high purity and high yield.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an extraction process of plant-derived gambogic acid, an active ingredient that inhibits the growth of tumor cells. Background technique [0002] Gambogic acid is a compound with high antitumor activity and good cell selectivity extracted from Garcinia cambogia. It has a low solubility in water and a large solubility in organic solvents such as alcohols and ketones. . Garcinia cambogia is a dry resin secreted by the Garcinia cambogia tree. At present, more than twenty gambogic acid compounds have been isolated and identified from garcinia cambogia. Commercially available garcinia cambogia is a cylindrical or irregular massive solid, brittle and brittle, with a yellow-brown and waxy luster. The solid is reddish-yellow or orange-brown, and the powder is bright yellow. Garcinia cambogia contains gambogic acid, new gambogic acid, and other components such as gambogic acid. [...

Claims

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Application Information

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IPC IPC(8): C07D493/20
Inventor 王洋徐利锋刘举陈烨周云鹏张秋实刘阳
Owner LIAONING UNIVERSITY
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