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Method for extracting resveratrol from grape leaves

A technology of resveratrol and grape leaves, which is applied in chemical instruments and methods, preparation of organic compounds, organic chemistry and other directions, can solve problems such as insufficient utilization of grape leaves, avoid low processing volume and high cost, The effect of alleviating the contradiction between supply and demand and avoiding insecurity problems

Inactive Publication Date: 2010-12-15
张波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a pity that in the past grape planting process, grape leaves were not well utilized as agricultural waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take the fresh grape leaves from the grape field, wash them and keep them away from light; take 80 parts of the grape leaves, add 6 parts of liquid nitrogen to pre-freeze for 10 minutes, and quickly crush them with a pulverizer after the leaves are embrittled; put the powder into 400 parts of concentration In 50% ethanol aqueous solution, soak for 16 hours; The resulting soaking solution is vacuum filtered to obtain a crude extract; the filter residue after vacuum filtration is mixed with 60% ethanol aqueous solution in a weight ratio of 1:2, Extract 3 times, each extraction time is 4 hours, vacuum filtration to remove the slag to obtain the second crude extract; combine the crude extract and the second crude extract to concentrate with a centrifugal vacuum thin-film evaporation concentrator; after the filtrate is properly concentrated, use Macroporous resin column: Purify by AB-8 column chromatography. After the solution is completely loaded, let it stand for 20 minutes...

Embodiment 2

[0029] Take the grape leaves from the grape field, wash and store them away from light, and then dry them in the shade; take 80 parts of the shade-dried grape leaves, add 7 parts of liquid nitrogen to pre-freeze for 15 minutes, and quickly crush them with a pulverizer after the leaves are embrittled; put the powder into 800 parts of concentration are in the ethanol aqueous solution of 70%, soak for 20 hours; Gained soaking liquid is passed through vacuum suction filtration, obtains crude extract; With the filter residue after vacuum suction filtration and concentration again, be the ethanol aqueous solution of 90% by weight ratio 1: 1 mixed, extracted 4 times, each extraction time is 5 hours, vacuum filtration to remove slag to obtain the second crude extract; combine the crude extract and the second crude extract to concentrate with a centrifugal vacuum thin-film evaporation concentrator; the filtrate is properly concentrated Finally, use a macroporous resin column: AB-8 colum...

Embodiment 3

[0031] Take the grape leaves that fall off naturally, wash them and store them away from light and dry them in the shade; take 80 parts of the shade-dried grape leaves, add 5 parts of liquid nitrogen to pre-freeze for 5 minutes, and quickly crush them with a pulverizer after the leaves are embrittled; put the powder into 200 parts of concentration In 40% ethanol aqueous solution, soak for 8 hours; The resulting soaking solution is vacuum filtered to obtain a crude extract; the filter residue after vacuum filtration is then mixed with 45% ethanol aqueous solution in a weight ratio of 1:3, Extract 2 times, each extraction time is 3 hours, and remove the slag by vacuum filtration to obtain the second crude extract; combine the crude extract and the second crude extract to concentrate with a centrifugal vacuum thin-film evaporation concentrator; after the filtrate is properly concentrated, use Macroporous resin column: Purify by AB-8 column chromatography. After the solution is com...

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Abstract

The invention provides a method for extracting resveratrol from grape leaves, comprising the steps of raw material pretreatment, extraction by using solvents and macroporous resin separation and purification. By using the grape leaves as raw materials, the invention provides a method for turning wastes into valuables for a grape industry, which can increase the incomes of peasants, reduce the agricultural production cost and also solve the conflict between the shortages of an increasingly reduced polydatin resource and the increase of a resveratrol demand. The method has simple operation without a precise instrument or automation equipment to avoid the dependence on the expensive raw materials and the precise instrument in an industrializing process, can be used for production in enterprises of rural areas with abundant grape resources and greatly reduce the production cost of the resveratrol; and liquid nitrogen as a freezing and crushing agent and alcohol as an extracting agent both have low toxicity and cheep prices, and a mature conventional technique for actual recovery can be utilized in the whole process, therefore, the environmental emission and the environmental pollution of extracted wastes are greatly reduced.

Description

technical field [0001] The invention relates to a method for extracting resveratrol from grape leaves. Background technique [0002] Resveratrol (resveratrol: trans-3,5,4'-trihydroxystilbene) is a stilbene compound (stilbenes), which exists in some medicinal plants and edible plants, such as cassia, veratrum, knotweed, grape, peanut. In 1992, Siemann and Creasy first confirmed that wine contains natural trans-resveratrol, and it has important medicinal effects on human liver, skin, heart, blood circulation and lipid metabolism. In recent years, it has also been found that resveratrol has special effects in anti-inflammation, cardiovascular protection, estrogen regulation and anti-tumor, and its biological activity has attracted widespread attention. [0003] At present, the source of resveratrol is mainly extracted from knotweed. The Chinese patents whose publication numbers are CN1321961C, CN101698634A, CN1566349A, CN1341587A, and CN101709024A adopt the method of extract...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/82
Inventor 张波王晓琴郑秋生黄方爱杨晓燕
Owner 张波
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