Method for detecting ox FTO (Fat Mass and Obesity-associated) gene single nucleotide polymorphism (SNP)

Abstract

The invention discloses a method for detecting ox FTO (Fat Mass and Obesity-associated) gene single nucleotide polymorphism (SNP), which comprises the following steps of: carrying out PCR (Polymerase Chain Reaction) amplification on an ox FTO gene by adopting FTO gene-contained ox whole genome DNA to be detected as a template and a primer pair P as a primer; after carrying out high-temperature denaturation on a PCR product, carrying out polyacrylamide gel electrophoresis; and identifying the SNP of the 783rd site (+1 as a translation initiation site) of an ox FTO gene coding area according toa polyacrylamide gel electrophoresis result. Because the FTO gene function involves in body length and body height characters, the detecting method provided by the invention lays the foundation for establishing an SNP and growth character relation of the FTO gene so as to be used for marker-assisted selection (MAS) of growth characters for Chinese beef and rapidly establish an ox population with favorable genetic resources.

Description

technical field [0001] The invention belongs to the field of molecular genetics, relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the single nucleotide polymorphism of cattle FTO gene. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the variation of a single nucleotide on the genome, forming genetic markers, and there are many of them. Includes substitutions, transversions, deletions and insertions. Theoretically speaking, a SNP may be a two-allelic polymorphism, or a three- or four-allelic polymorphism, but in practice, the latter two are very rare and can almost be ignored. Therefore, the so-called SNPs are biallelic polymorphisms. This variation may be a transition (C, T, and G, A on its complementary strand) or a transversion (C-A, G-T, C-G, A-T). The incidence of conversion is always significantly higher than that of several other variants, the SNPs with conversion variants acc...

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Problems solved by technology

So far, there has been no research on the genetic variation of the FTO gene in cattle at home and abroad. Due to the lack of research on the genetic variation of the FTO gene in Chinese cattle, the functional research of this gene locus and its genetic variation and economic traits (such as: body weight, body height, body weight, etc.) Long-term traits) association research is still blank

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