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Method for preparing silk fibroin three-dimensional porous material

A technology of silk fibroin and porous material, which is applied in medical science, prosthesis, etc., can solve the problem that the proportion of silk fibroin I and silk fibroin II cannot be adjusted, and achieve the effect of good structure

Active Publication Date: 2010-12-08
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to provide a method for preparing a porous three-dimensional silk fibroin material, which overcomes the defect of the separated sheet structure in the porous three-dimensional silk fibroin material obtained in the prior art, and the silk I in the three-dimensional silk fibroin porous material obtained Defects in which the ratio of the silk fibroin II structure cannot be adjusted

Method used

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  • Method for preparing silk fibroin three-dimensional porous material
  • Method for preparing silk fibroin three-dimensional porous material
  • Method for preparing silk fibroin three-dimensional porous material

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0039] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0040] Take 15mL of the above-mentioned silk protein solution, and adjust the concentration speed by reducing the volatilization area of ​​the solution under the condition of 60°C. After concentration for 48 hours, the concentration is about 30%. After concentration, an aqueous solution of silk fibroin nanowires is obtained...

Embodiment 2

[0043] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0044] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0045] Take 15mL of the above silk protein solution, and adjust the concentration speed by reducing the volatilization area of ​​the solution under the condition of 60°C. After concentration for 48 hours, the concentration is about 30%. After concentration, the aqueous solution of silk fibroin nanowires is obtained; the sol...

Embodiment 3

[0049] Soak about 50g raw silk in 2L 0.5% Na 2 CO 3 solution, stirred and boiled for 30 minutes, then removed and washed with deionized water. After repeating the above operation twice, the silk was dried at 60°C.

[0050] 15 g of the degummed silk after the above treatment was weighed and dissolved in 100 ml of BrLi solution with a concentration of 9.5 mol / L, stirred and dissolved at 60° C. for one hour. Then use a dialysis bag with a molecular weight cut-off of 3500 to dialyze with deionized water for four days to obtain a silk protein aqueous solution with a concentration of 4%, and adjust the concentration of the silk protein aqueous solution to 2%.

[0051] Take 15mL of the above silk protein solution, and adjust the concentration speed by reducing the volatilization area of ​​the solution under the condition of 60°C. After concentration for 48 hours, the concentration is about 30%. After concentration, the aqueous solution of silk fibroin nanowires is obtained; the sol...

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Abstract

The invention relates to a porous material and a method for preparing the same and discloses a method for preparing a silk fibroin three-dimensional porous material. The method comprises the following steps of: slowly concentrating a water solution of silk fibroin, uniformly mixing the water solution of silk fibroin with alcohol, and freezing and drying the misture to directly obtain a water-insoluble silk fibroin porous support material; regulating and controlling the secondary structure and the mechanical properties of the porous support by selectively using after-treatment means, and controlling the porosity, the pore diameter and the like of the porous material by changing the parameters of silk fibroin concentration, freezing temperature and the like; and finally, soaking the silk fibroin porous support material in water to remove alcohol. The invention has mild preparation process conditions, no addition of any cross-linking agent, any hole making agent and other toxic organic solvents, simple, convenient and controllable process and easy realization of industrialization; the biocompatibility of silk fibroin can be effectively maintained, and the silk fibroin can be applied to the tissue repair of bone, cartilage, anadesma, nerves, skin and the like, the drug controlled-release carriers and the like.

Description

technical field [0001] The invention relates to a porous material and a preparation method thereof, in particular to a technique for preparing a porous three-dimensional material using silk fibroin as a raw material. The prepared material can be applied to technical fields such as biomedicine, biotechnology, and tissue engineering. Background technique [0002] Biomedical materials, especially tissue engineering scaffold materials, tissue repair regeneration materials, etc., require the materials used not only to have good biocompatibility, but also to have a suitable three-dimensional porous structure, controllable biodegradability, and certain mechanical strength. , and the ability to release factors or other related drugs. [0003] For the method of preparing biomedical materials, especially the method of preparing porous scaffolds of biomaterials, it is required that the preparation process should be as gentle as possible, and the use of toxic organic solvents should be ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/56
Inventor 张冰吕强李明忠卢神州王建南
Owner SUZHOU UNIV
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