Cultivating method of zostera aseptic seedlings

Abstract

The invention relates to a cultivating method of zostera aseptic seedlings, comprising the following steps of: preserving collected zostera seeds for a month or longer at a low-temperature condition of 4 DEG C to break a seed resting state by low-temperature stimulation; then, cleaning the zostera seeds preserved at low temperature by sterilized seawater under an aseptic condition; picking strong and perfect seeds; sterilizing the seeds for 30s by using ethanol of 70 percent; sterilizing the seeds for 10min by using 10 percent sodium hypochlorite; planting the seeds into a conical bottle with an MS liquid culture medium after cleaning the seeds by the sterilized seawater; cultivating the conical bottle into an illumination cultivating box under the cultivating conditions with the temperature of 22 DEG C, the illumination intensity of 1000Lx and the illumination time of 12h; and changing the culture medium once every four days until the length of the seedlings is 2-3 cm. The cultivating method can be used for cultivating at all seasons, ensures the seedling supply in zostera scale cultivation, and is also suitable for aseptic seedling cultivation of other seedlings of zostera L.of zosteraceae of helobial.

Description

technical field [0001] The invention belongs to the field of cultivating seaweed seedlings, and in particular relates to a method for cultivating aseptic seedlings of eelgrass. Background technique [0002] Eelgrass is a higher aquatic seaweed plant. Due to its special structure, it not only has the growth characteristics of aquatic plants, but also has the growth characteristics of terrestrial plants. In recent years, due to the change of environmental conditions and the interference of human factors, the resources of eelweed have been greatly reduced. In order to realize its sustainable utilization, the establishment of tissue culture technology can reproduce a large number of algae in a short period of time, which is very important for the protection and rational development and utilization of eel algae. Algae has important practical significance, providing a healthy environment for mariculture, and achieving a win-win situation for mariculture and ecological restoration....

AI Technical Summary

Problems solved by technology

[0004] The problems and deficiencies in the above-mentioned prior art are that, in "A Method for Growing Saffron Aseptic Vaccines", callus is selected as explants to induce aseptic vaccines, and the chromosomal ploidy of the callus varies greatly and is unstable; For seaweed, the technology of inducing callus is immature, the callus induction rate is extremely low, and the pollution rate is high, so it is of little practical significance to select callus as explants to cultivate sterile vaccines

"Tissue Culture of Aseptic Seedlings of Anthurium and Transplanting Techniques of Test-tube Seedlings" and "Method of Cutting Aseptic Seedlings of Hippeastrum" use the organs of the plant for the cultivation of aseptic seedlings, and it is difficult to ensure a relatively tender cultivation Tissues, the direct damage of disinfectants to young tissues is unavoidable; the cell wall of eel algae is very thin, and the direct damage of disinfectants to cells is much more serious than that of terrestrial higher plants, so it is difficult to guarantee the survival rate of culture

"A method for rapid germination of pokeweed seeds and its application in the cultivation of sterile seedlings", soaking pokeweed seeds with concentrated sulfuric acid to achieve disinfection and break the dormancy of seeds, although eelgrass has the effect of terrestrial plants Growth characteristics, but not as advanced as terrestrial plants, and the seed structure is not as perfect as pokeweed seeds, so the harmful effect of concentrated sulfuric acid on the seeds cannot be ignored, and a relatively mild disinfectant should be selected to achieve the purpose of disinfection