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Plant drought-resistance and salt-tolerance associated protein TaNAC, and encoding gene and application thereof

A technology related to proteins and plants, applied in the field of genetic engineering, can solve problems such as low yield and growth retardation

Inactive Publication Date: 2010-12-01
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression of rice OsNAC6 is not only induced by abiotic stresses such as drought, low temperature, and high salinity, but also induced by mechanical damage and rice blast. Rice overexpressing this gene has improved tolerance to drought, high salt, and rice blast , but showed growth retardation and low yield

Method used

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  • Plant drought-resistance and salt-tolerance associated protein TaNAC, and encoding gene and application thereof
  • Plant drought-resistance and salt-tolerance associated protein TaNAC, and encoding gene and application thereof
  • Plant drought-resistance and salt-tolerance associated protein TaNAC, and encoding gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Embodiment 1, cloning and sequence motif analysis of TaNAC gene

[0073] 1. The wheat Jingdong 17 plants cultivated under light at 25°C for about 2 weeks were subjected to drought and high salt (200mM NaCl) stress treatments, samples were taken after 0h, 1h, 2h, 5h, 10h, and 24h, and quickly placed in liquid nitrogen to freeze , stored at -80°C for later use. According to the RNA extraction kit (Beijing Tiangen Biochemical Technology Co., Ltd.), the total RNA extraction of plant leaf tissue was completed, and then the extracted total RNA was used as a template, and the AP sequence was used as a primer, and reverse transcriptase (M-MLV) was used for reverse transcriptase. Transcribed to obtain cDNA templates for subsequent experiments.

[0074] Through the rice NAC sequence, use tBLSATN to search the wheat EST library, obtain the full-length cDNA sequence of the gene through analysis and comparison, and design a pair of primers by danman: TaNAC-F1: 5′-AGG ACC ACT

[00...

Embodiment 2

[0080] Example 2. Gene expression characteristics of TaNAC gene under drought and high-salt adversity stress treatment

[0081] Wheat Jingdong 17 plants cultivated under light at 25°C for about 2 weeks were subjected to drought and high salt (200mM NaCl) stress treatments, and samples were taken after 0h, 1h, 2h, 5h, 10h, and 24h, and quickly placed in liquid nitrogen to freeze,- Store at 80°C for later use. Total RNA was extracted from plant leaf tissue according to the RNA extraction kit (Beijing Tiangen Biochemical Technology Co., Ltd.).

[0082] Wheat actin (GeneBank accession number AB181991) was selected as the internal reference gene in this experiment. Internal reference gene amplification primers actin-F: 5′-CGG CTA CCA CAT CCAAGG AA-3′; actin-R: 5′-GCTGGA ATT ACC GCG GCT-3'. Design primers from the non-conserved sequence of the target gene, control the length of the amplified fragment as close as possible to the length of the internal reference gene, target gene am...

Embodiment 3

[0086] Example 3, identification of drought tolerance and salt resistance of transgenic TaNAC tobacco

[0087] The TaNAC gene was linked to the downstream of the 35S promoter of the PBI121 binary plant expression vector to construct an overexpression vector, and transformed into tobacco W38 strain by Agrobacterium tumefaciens C58C1-mediated transformation of tobacco leaf discs. Transgenic tobacco plants that are positive in the PCR test grow to a certain height and then transplanted to flower pots, cultivated under 16 hours of light at 24°C, harvested the seeds after about 5 months, and then treated with simulated drought and salt stress, and the sterilized seeds Seed in MS medium containing 2% PEG and 200mM NaCl, observe the growth of transgenic seedlings and wild-type control.

[0088] The results of the drought resistance experiment show that the growth of the seeds two weeks after germination is as follows: figure 2 A, The left half of the plate is the transgenic line, a...

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Abstract

The invention relates to the field of gene engineering, in particular to a plant drought-resistance and salt-tolerance associated protein TaNAC, an encoding gene thereof and application thereof. The amino acid sequence of the protein is shown as SEQ ID NO.1 and the gene sequence is shown as SEQ ID NO.2. The plant drought-resistance and salt-tolerance associated protein and the encoding gene thereof of the invention has very important theoretical and practical significance for improving and enhancing the stress resistance of tobacco, increasing the yield, promoting the breeding process of stress resistant molecules and effectively saving water resources.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular, the invention relates to a plant drought-resistant and salt-tolerant related protein TaNAC and its coding gene and application. Background technique [0002] Adversity stresses such as drought and salinity are the main abiotic stress factors that limit plant growth and crop yield. According to statistics, the world's arid and semi-arid areas account for one-third of the global land area, and my country's arid and semi-arid areas account for one-half of the country's land area; there are 3.6×107hm 2 The saline-alkali land needs to be reclaimed and utilized. As one of the important food crops in our country, wheat occupies a very important position in the national economy. However, due to adversity such as drought and salinity, the production of wheat in my country is reduced by 70-80 billion kilograms every year, seriously affecting the yield and quality of wheat, and restrictin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63A01H5/00
Inventor 唐益苗赵昌平高世庆冶晓芳刘美英张朝
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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