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Method for preparing bioethanol by utilizing kelp processing waste

A bioethanol and waste technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of environmental pollution of kelp processing waste, achieve low cost, simple process, and solve environmental pollution problems Effect

Inactive Publication Date: 2010-11-10
THE FIRST INST OF OCEANOGRAPHY SOA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing bioethanol by using kelp processing waste to make up for the deficiencies in the prior art, solve the environmental pollution problem of kelp processing waste, and break through the main bottleneck of bioethanol industrialization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1 (wherein with trichoderma viride hydrolysis saccharification pretreatment liquid)

[0016] Preparation of bioethanol consists of the following steps:

[0017] (1) Pretreatment of kelp processing waste: wash the kelp processing waste with water, soak for 24 hours, homogenize for 10 minutes, and then sterilize at 121° C. for 30 minutes to obtain a pretreatment solution;

[0018] (2) Hydrolysis and saccharification of pretreatment liquid: use Mandel's inorganic salt nutrient solution, at temperature 35 ℃, pH6, cultivate Trichoderma viride for 48h, add the activated Trichoderma viride fermentation liquid according to its access volume ratio of 20% into the pretreatment solution, the fermentation temperature is 50°C, the pH is 6, and the saccharification solution is obtained after hydrolysis and saccharification for 48 hours;

[0019] (3) Preparation of bioethanol by fermentation of saccharification liquid: use yeast basic medium at a temperature of 35°C and pH...

Embodiment 2

[0021] Embodiment 2 (wherein hydrolysis and saccharification pretreatment liquid with cellulase)

[0022] Preparation of bioethanol consists of the following steps:

[0023] (1) Pretreatment of kelp processing waste: wash the kelp processing waste with water, soak for 20 hours, homogenize for 10 minutes, and then sterilize at 121° C. for 30 minutes to obtain a pretreatment solution;

[0024] (2) Hydrolysis and saccharification of the pretreatment solution: add 1 g / L cellulase preparation to the pretreatment solution, the hydrolysis and saccharification temperature is 50° C., pH 7, and the hydrolysis and saccharification time is 48 hours to obtain the saccharification solution;

[0025] (3) Preparation of bioethanol by fermentation of saccharification liquid: use yeast basic medium at a temperature of 35°C and pH 5 to activate Saccharomyces cerevisiae strains; add the activated Saccharomyces cerevisiae fermentation liquid to step (2) according to the volume ratio of 15% Carry ...

Embodiment 3

[0027] Embodiment 3 (wherein with trichoderma viride and cellulase hydrolysis saccharification pretreatment liquid together)

[0028] Preparation of bioethanol consists of the following steps:

[0029] (1) Pretreatment of kelp processing waste: wash the kelp processing waste with water, soak for 24 hours, homogenize for 10 minutes, and then sterilize at 121° C. for 30 minutes to obtain a pretreatment solution;

[0030] (2) The hydrolysis and saccharification of the pretreatment liquid: be 10% by volume ratio and add Mandel's inorganic salt nutrient solution, at temperature 35 ℃, pH6, the Trichoderma viride activated for 48h and by weight volume ratio 0.5g / L is added with cellulase hydrolysis and saccharification pretreatment solution, the hydrolysis and saccharification temperature is 50°C, pH 6, and the saccharification solution is obtained after hydrolysis and saccharification for 48 hours;

[0031] (3) Fermentation of saccharification liquid to produce bioethanol: use yea...

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Abstract

The invention relates to a method for preparing bioethanol by utilizing kelp processing waste. The method comprises the following steps: firstly cleaning the kelp processing waste, homogenizing, sterilizing and preparing p pretreatment liquid; further carrying out hydrolysis saccharification on the pretreatment liquid: adding activated trichoderma viride fermentation liquid according to the volume ratio of 5-20% or / and adding cellulase according to the weight-volume ratio of 0.5-1g / L for carrying out the hydrolysis saccharification on the pretreatment liquid, wherein the temperature for the hydrolysis saccharification is 45-55 DEG C, the pH is 5-7 and the time is 36-48h; further adding well activated saccharomyces cerevisiae zymocyte into liquid after the hydrolysis saccharification according to the volume ratio of 10-20% for fermentation, and preparing ethanol, wherein the fermentation temperature is 25-35 DEG C, the pH is 4-6 and the fermentation time is 50-60h; and carrying out liquid-solid separation on the fermentation liquid after the fermentation is finished, and evaporating out the ethanol from supernatant fluid in the reduced pressure distillation method. The method has simple process and low cost, and can not only solve the environmental pollution problem of the kelp processing waste, but also provide a new material source for large-scale production of the ethanol and promote the development of marine biomass energy.

Description

technical field [0001] The invention belongs to the technical field of marine resource biology, and in particular relates to a method for preparing bioethanol by using kelp processing waste. Background technique [0002] At present, the world is facing the huge pressure of energy shortage and environmental pollution, and the development of clean and renewable energy has become an important means to solve the crisis. Bioethanol has been widely concerned as a clean fuel and gasoline additive. However, the increasingly severe world food situation has made the first-generation bioenergy, which uses corn, wheat and other food crops as raw materials, gradually lose its advantages. Biofuels (represented by non-grain crop ethanol, cellulosic ethanol and biodiesel, etc.) are becoming the direction of future bioenergy industry development. At present, the preparation of fuel ethanol in my country mainly focuses on terrestrial raw materials such as straw, hay, bagasse, rice husk, saw...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/865
CPCY02E50/16Y02E50/10
Inventor 缪锦来郑洲
Owner THE FIRST INST OF OCEANOGRAPHY SOA
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