Method for collecting semen of primates of macaque by electro stimulation

Abstract

The invention provides a method for collecting semen of primates of macaque by electro stimulation, belonging to the method of animal reproduction, in particular to the method for collecting sperm of primates of macaque. In the invention, aluminium foil electrodes coated by absorbent cotton infiltrated by saline encircles around the penis of the anesthetized animal and is connected with the impulse power; the voltage begins from 0V, and stimulation is conducted for 10-15s continuously when each 10V is increased, until the voltage reaches 60V; the process is circulated for 6 to 7 times when necessary, with an interval of 20-30s between two stimulations; and the collected semen can be used for fertilization after incubation and separation. The invention can improve the success rate for collecting semen of animals, and has no pollution; the semen collected in the invention has similar index as that of the semen gained through natural mating and can be applied to external fertilization and artificial fertilization.

Description

Technical field [0001] The invention belongs to an animal reproduction method, in particular a method for collecting sperm from a primate of the macaque genus. Background technique [0002] Macaques are primates, such as rhesus monkeys, cynomolgus monkeys, bear monkeys, Tibetan chief monkeys, etc., which are close relatives of humans. Their tissue structure, physiology, biochemistry, immunity and metabolism are highly similar to humans, and they are responsible for infectious diseases such as viruses. The best experimental animal for research on models, neuropsychiatric disease models, cardiovascular disease models, tumor models and reproductive physiology. However, due to the continuous deterioration and destruction of the wild living environment, the reproductive capacity and necessary conditions of wild primates have gradually declined and lost. The primate species in the Macaca genus have become rare and endangered animals and have been listed as national first and second pro...

AI Technical Summary

Problems solved by technology

The rectal stimulation method needs to flush and remove the feces in the rectum of the animal before semen collection. The electrode needs to be accurately placed on the prostate during collection. This part is easy to stimulate the bladder and other organs and cause the semen to be polluted by urine. The method is cumbersome and unhygienic.

Conventional primate penile electrical stimulation methods use aluminum foil, copper mesh, etc. to directly wrap the penis of the animal, and the metal electrode is in direct contact with the skin. Due to the different structure of the penis of this type of animal, the contact status between the penis and the electrode and the skin impedance of the penis are different. There are dense spiny horny papilla near the tip of the glans and the dorsal side of the back edge of the penis of Tibetan monkeys and bear monkeys. It is almost impossible to make the metal electrodes evenly contact the surface of the penis, and it is easy to generate local excessive current to burn the epidermis of the penis and even the epidermis. damage