Recombinant human FSHR-57 polypeptide protein and preparation method and application thereof

Abstract

The invention relates to recombinant human FSHR-57 polypeptide protein and a preparation method and application thereof. The polypeptide vaccine selects N-terminal 57-site amino acid of follicle stimulating hormone receptor (FSHR), contains B cell epitopes and effectively avoids crossing reaction with luteinizing hormone receptor (LHR) and thyroid stimulating hormone receptor (TSHR). The high-purity FSHR-57 polypeptide protein is prepared by using methods of prokaryocyte expression, rubber cutting purification and gradient dialysis renaturation. Non-reduced PAGE + Western blot, HPLC and mass spectrometry amino acid sequencing identification verify that the polypeptide protein prepared by the method is accurate and has the purity of higher than 97 percent. The FSHR-57 polypeptide protein has good safety, does not disturb the level of sex hormone such as testosterone, estradiol and the like, has stronger immunogenicity, and can become a candidate of male contraceptive vaccine.

Description

1. Technical field [0001] The invention belongs to the field of bioengineering, and specifically relates to a recombinant human FSHR-57 polypeptide protein for male contraception, its preparation method and pharmaceutical application. 2. Background technology [0002] With the rapid growth of the world population, the task of population control is more difficult, but in the past most of the responsibilities were borne by women, and the development of society requires men to play an active role. [0003] Male contraceptives under development are divided into hormonal contraceptives and non-hormonal contraceptives (external spermicides, Chinese herbal medicines and contraceptive vaccines). However, no matter whether male hormone contraceptives are used alone or in combination, there are still many unsatisfactory aspects to be improved. Various non-hormonal contraceptives also have shortcomings: spermicides are prone to allergic reactions, and the effect is not satisfactory wh...

AI Technical Summary

Problems solved by technology

Various non-hormonal contraceptives also have shortcomings: spermicides are prone to allergic reactions, and the effect is not satisfactory when used alone; Chinese herbal medicines have large toxic and side effects, complex ingredients and unclear mechanisms, and are currently only used in animal experiments

There is also a lack of in-depth and systematic research on the contraceptive mechanism and safety evaluation

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