Crystallizing method of 5'-guanosine-monophosphate disodium salt

Abstract

The invention discloses a crystallizing method of a 5'-guanosine-monophosphate disodium salt, which has the advantages of simple operation as well as large crystalline particle, uniform particle, attractive fineness and high purity of products. The method comprises the following steps of: adding sodium hydroxide to a 5'-GMP aqueous solution of which the concentration is 5-40 percent; regulating the pH value of the solution to 7-11 and then adding absolute alcohol which has a pH value of 7-11 and is 0.5-3 times of the solution in volume and a catalyst; stirring at 15-80 DEG C to separate out 5'-GMP, 2Na; and centrifuging, washing and drying to obtain 5'-GMP, 2Na crystals, wherein the catalyst is a mixture of a 60-120 mesh siftage of 5'-guanosine-monophosphate disodium salt crystals and sodium carbonate, the mass ratio of the 60-120 mesh siftage of 5'-guanosine-monophosphate disodium salt crystals to the sodium carbonate is 1:2, and the addition quantity of the catalyst is 0.1-0.2 percent of the mass of the 5'-GMP.

Description

Technical field: [0001] The invention relates to a crystallization purification method, in particular to a crystallization method of 5'-guanosine-phosphate disodium salt with simple operation, large product crystallization, good fluidity, uniform particles, beautiful color and high purity. Background technique: [0002] 5'-nucleotides (CMP, AMP, UMP, GMP, IMP) are products obtained by enzymatic degradation of RNA, and have been widely used in the fields of medicine, chemical industry, food, health care products and agriculture, such as the international infant milk powder industry The standard stipulates that 5'-nucleotides must be added, and the purity of 5'-nucleotides has always been a difficult problem in industrial production. [0003] At present, the known purification method of 5'-guanosine-phosphate disodium salt (5'-GMP, 2Na) is a conventional crystallization method, i.e. ethanol elution method. First use sodium hydroxide to adjust a certain concentration and tempe...

AI Technical Summary

Problems solved by technology

The ethanol solvent reduces the solubility of 5'-GMP and 2Na in the solution, resulting in the violent precipitation of 5'-GMP and 2Na. The precipitated product is a white powder or small crystal with high viscosity, and it is difficult to separate the solid and liquid by centrifugation.

The obtained product has problems such as uneven particles, strong hygroscopicity, easy clumping and difficulty in sieving, low purity and poor fluidity.