Sepiella maindroni releasing and tagging chip and detection method
A Mansfield needle-free squid, flow labeling technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of data analysis error, large damage to squid, loss of labeling stability, etc., and achieve statistical data analysis. The effect of reduced error rate, good specificity and stability, and less damage
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[0022] 1. Establish a high-throughput detection system for released populations
[0023] Using capillary electrophoresis SSR fluorescent labeling, a high-throughput EST-SSR labeling detection system for squid release populations was established in terms of rapid DNA extraction, PCR amplification, and PCR product detection. The cDNA fingerprint library of the population, as shown in the two Oligo DNAs of the present invention: SEQ ID NO.1 (number: Tai-2009-1) and SEQ ID NO.2 (number: Zhou-2009-1), was obtained by screening Specific fingerprinting of squid populations.
[0024] 1.1 Extract the DNA of each squid population and perform PCR reaction. Reaction program: 94°C pre-denaturation for 5 minutes, 30 cycles (94°C denaturation for 30s; appropriate temperature annealing for 30s; 72°C extension for 45s), 72°C extension for 10 minutes .
[0025] 1.2 Dilute the PCR product 30 times, add 9.05 μL deionized formamide, 0.05 μL GS3730_500 molecular weight internal standard and 1 μL ...
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