Sepiella maindroni releasing and tagging chip and detection method

A Mansfield needle-free squid, flow labeling technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of data analysis error, large damage to squid, loss of labeling stability, etc., and achieve statistical data analysis. The effect of reduced error rate, good specificity and stability, and less damage

Inactive Publication Date: 2010-10-13
NINGBO UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

There are great difficulties in the application of the above methods to the research on the discharge marking of squid. At present, the release of squid is still a simple mark using the scissor arm. This method can only identify the individual squid that is marked, and cannot identify the squid that is the parent. The marking of the population, and the regeneration of the tentacles will lose the stability of the marking, so there are large errors in the data analysis after the capture, and the damage to the squid is also large

Method used

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Embodiment

[0022] 1. Establish a high-throughput detection system for released populations

[0023] Using capillary electrophoresis SSR fluorescent labeling, a high-throughput EST-SSR labeling detection system for squid release populations was established in terms of rapid DNA extraction, PCR amplification, and PCR product detection. The cDNA fingerprint library of the population, as shown in the two Oligo DNAs of the present invention: SEQ ID NO.1 (number: Tai-2009-1) and SEQ ID NO.2 (number: Zhou-2009-1), was obtained by screening Specific fingerprinting of squid populations.

[0024] 1.1 Extract the DNA of each squid population and perform PCR reaction. Reaction program: 94°C pre-denaturation for 5 minutes, 30 cycles (94°C denaturation for 30s; appropriate temperature annealing for 30s; 72°C extension for 45s), 72°C extension for 10 minutes .

[0025] 1.2 Dilute the PCR product 30 times, add 9.05 μL deionized formamide, 0.05 μL GS3730_500 molecular weight internal standard and 1 μL ...

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Abstract

The invention discloses a sepiella maindroni releasing and tagging chip and a detection method. The chip comprises a quality-control probe and a detection probe, wherein the quality-control probe comprises an immobilized positive probe, a hybrid positive probe, a positive contrast probe and a negative contrast probe which are artificially synthesized; the detection probe is the nucleotide sequence of two Tai-2009-1 or Zhou-2009-1 releasing groups with high specialty; the releasing and tagging chip can be used for identifying the group taking the two sepias as parents. The releasing and tagging chip has the advantages of bringing convenience to the research of the correlation between the sepiella maindroni releasing groups and descendants and particularly providing a precise data basis for the planning committee for the research of the growth and reproduction of the sepias, along with high tagging specialty and stability, little damage to the sepias and reduced analysis error rate of statistical data of each recaptured group.

Description

technical field [0001] The invention relates to a needleless cuttlefish release mark, in particular to a needleless cuttlefish discharge mark chip and a detection method. Background technique [0002] Squid (Sepiella maindroni, Rochebrune), commonly known as squid, cuttlefish, cuttlefish, sea cat, etc., belongs to the mollusk phylum, cephalopods, decabranches, and squid family. Due to the habit of inkjet and poor resistance to dry dew , have been difficult to feed for a long time, few people have seen live cuttlefish, and it is a worldwide problem to study the biology of cuttlefish and its nursery and breeding techniques with living bodies. The inventor caught 37 live squids in the natural sea area, not only fed the squids, but also made them lay eggs normally through certain measures, and then carried out artificial breeding and breeding research, and obtained breakthrough progress. More than 7,300 squid seedlings have been bred, and 1,008 finished squids have been raised,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 宋微微王春琳母昌考
Owner NINGBO UNIV
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