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Vaccine for preventing porcine reproductive and respiratory syndrome

A respiratory syndrome and vaccine technology, applied in the field of vaccines for the prevention of porcine reproductive and respiratory syndrome, can solve problems such as amino acid deficiency and inability to protect

Inactive Publication Date: 2012-01-04
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the variation of the virulence of the strain, a new popular form of PRRS appeared in the United States in 1997, that is, acute and atypical PRRS, which caused higher abortion rates and mortality in sows. Traditional vaccines such as traditional vaccines cannot fully protect the infection of these emerging virulent strains of PRRSV. In the outbreak of the disease in southern my country in 2006, studies have shown that amino acids 483 and 535-563 in the Nsp2 sequence are missing

Method used

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  • Vaccine for preventing porcine reproductive and respiratory syndrome
  • Vaccine for preventing porcine reproductive and respiratory syndrome
  • Vaccine for preventing porcine reproductive and respiratory syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the preparation of the vaccine that prevents porcine reproductive and respiratory syndrome

[0026] 1. Design of NSP gene and SP gene

[0027] 1. Design of NSP gene

[0028] Select 4 relatively conserved epitopes (BCE1-4) among the B cell epitopes of PRRSV non-structural protein Nsp2; The last universal T cell epitope PADRE (TCE); these 6 epitopes are connected by the three amino acid linking sequence GGS to form the protein sequence of the multi-epitope subunit vaccine NSP (see Table 1 for the amino acid sequence of each epitope).

[0029] Table 1 Protein sequence of subunit vaccine NSP

[0030]

[0031] Note: BCE is B cell epitope, TCE is T cell epitope

[0032] According to the base codon preference of Escherichia coli, use DNAstar software to generate NSP nucleic acid sequence, design primers as shown in Table 2 (introduce BamHI restriction site at the N-terminus, introduce XhoI restriction site at the C-terminus), and send to Shanghai Bioenginee...

Embodiment 2

[0107] Embodiment 2, the preparation of the vaccine that prevents porcine reproductive and respiratory syndrome

[0108] The method of Step 2 and Step 3 of Example 1 was used again to prepare a vaccine to obtain a vaccine against porcine reproductive and respiratory syndrome (batch number 0912002).

Embodiment 3

[0109] Embodiment 3, the preparation of the vaccine that prevents porcine reproductive and respiratory syndrome

[0110] The method of Step 2 and Step 3 of Example 1 was used again to prepare a vaccine to obtain a vaccine against porcine reproductive and respiratory syndrome (batch number 0912003).

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Abstract

The invention discloses a vaccine for preventing porcine reproductive and respiratory syndrome. The vaccine provided by the invention comprises active ingredients consisting of NSP polypeptide and SP polypeptide, wherein an amino acid sequence of the NSP polypeptide is shown in the sequence 3 in a sequence table; and the amino acid sequence of the SP polypeptide is shown in the sequence 1 in the sequence table. The vaccine is prepared by uniformly emulsifying the NSP polypeptide, the SP polypeptide and a Freund adjuvant, and has the characteristics of application to the prevention of the porcine reproductive and respiratory syndrome, security, effectiveness, low cost, easy preparation and suitability for large-scale popularization and application.

Description

technical field [0001] The invention relates to a vaccine for preventing porcine reproductive and respiratory syndrome. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a contagious disease discovered in the late 1980s, which can cause reproductive disorders such as abortion, stillbirth, and mummified fetuses in pregnant sows, as well as various ages. Respiratory diseases of pigs (especially piglets). The occurrence of the disease was first reported in the United States in 1987, and then rapidly spread to countries and regions with developed swine industry in the world (North America, 1987; Europe, 1990; Asia, 1991). The Central Veterinary Research Institute of the Netherlands (Lelystad et, 1991) isolated the pathogen for the first time from porcine alveolar macrophages and named it Lelystad virus. In 1992, American researchers also isolated the American representative strain of PRRSV (VR2332) using CL2621 cells. In the same year, the E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/12C12N15/40C12N15/63C12N1/21C07K14/08A61P31/14C12R1/19
Inventor 刘文军陈才伟孙蕾李晶
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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