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Process for concentrated biomass saccharification

A technology for biomass and pretreatment of biomass, which is used in the field of saccharification for concentrated biomass, and can solve the problems of difficulty in completing saccharification of biomass.

Active Publication Date: 2010-09-29
EI DU PONT DE NEMOURS & CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, biomass saccharification with high sugar yields at such high biomass concentrations in economically viable reactor systems has remained elusive.

Method used

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  • Process for concentrated biomass saccharification
  • Process for concentrated biomass saccharification
  • Process for concentrated biomass saccharification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] Saccharification of pretreated biomass with and without stirring

[0132] Two productions (#25 and 26) were carried out with ground pretreated biomass at 50°C, pH = 5.5, with a feed of about 20% DWB (dry weight of biomass). The biomass used in these production runs was a blend of three batches of pretreated corncobs reduced in particle size to about 1 mm. In the steam injection reactor as described above, by using 4g NH 3 A batch labeled HT-4 was prepared by treating broken cobs with steam at 145°C for 20 minutes per 100 g dry weight of biomass. Two additional batches were prepared by treating corn cobs with 6 g NH3 per 100 g dry weight of biomass and steam at 145 °C for 10 min in a barrel piston reactor as described above. The blend of these three batches of pretreated biomass was ground in a Waring commercial blender and sieved through a 1.1 mm sieve before being used in the saccharification experiments.

[0133] Production was performed in a 500 mL glass-jackete...

Embodiment 2

[0143] Saccharification of pretreated biomass with different initial sizes

[0144] Production runs 50 to 52 and 64 to 65 were performed with pretreated biomass ground to different sizes. Pretreated biomass was prepared from ground corncobs that were hammer milled and sieved through a 1 / 2 inch screen. They were in a Jaygo reactor as described in General Methods with 4 g NH 3 Steam treatment at 145 °C for 20 min per 100 g dry weight of biomass. This pretreated corncob biomass was labeled Jaygo-10. Prior to saccharification, the pretreated cobs are further ground and screened through appropriately sized screens in multiple steps to prepare the biomass for each saccharification production run. The screen sizes used to prepare the samples for each production run are shown in Table 2 below.

[0145] Production runs 50 and 51 were performed in 2-L reactors, and production runs 52, 64 and 65 were performed in 0.5-L reactors. In all cases, deionized water was used as the reacti...

Embodiment 3

[0156] Biomass liquefaction during enzymatic saccharification

[0157] As noted above, production run 52 may be used to measure changes in particle size distribution and liquefaction of biomass as the saccharification process continues. After 6 hours and 72 hours, the particle size distribution of the corncob biomass and hydrolyzate subjected to the initial grinding pretreatment was measured.

[0158] Table 4: Particle size distribution reduction during saccharification-production run 52

[0159]

[0160] The results clearly indicate that enzyme-catalyzed saccharification reduces the particle size distribution of the solids, thus causing liquefaction of the solids, allowing additional solids to be added to the hydrolyzate.

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Abstract

Processes for saccharification of pretreated biomass to obtain high concentrations of fermentable sugars are provided. Specifically, a process was developed that uses a fed batch approach with particle size reduction to provide a high dry weight of biomass content enzymatic saccharification reaction, which produces a high sugars concentration hydrolysate, using a low cost reactor system.

Description

[0001] Statement of Government Rights [0002] This invention was made with US Government support and is made subject to Contract Nos. 04-03-CA-70224 and DE-FC36-03GO13146 with the Department of Energy. The United States Government has necessary rights in this invention. field of invention [0003] Methods for saccharification of pretreated biomass to obtain high concentrations of fermentable sugars are provided. Specifically, a method was developed that uses a fed-batch process with particle size reduction to provide high dry weight biomass content in an enzyme-catalyzed saccharification reaction that uses a low-cost reactor system to produce high sugar concentration of hydrolyzate. Background of the invention [0004] Cellulosic and lignocellulosic raw materials, as well as waste such as agricultural residues, wood, forestry waste, sludge from the paper industry, and municipal and industrial solid waste, offer a huge potential for renewable raw materials for the product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02C12P7/08
CPCY02E50/17Y02E50/16C12P7/00C12P19/02C12P19/14C12P7/10Y02E50/10
Inventor S·M·亨尼西M·西潘R·T·埃兰德M·P·塔克三世
Owner EI DU PONT DE NEMOURS & CO
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