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Method for separating and purifying natural acteoside

A technology for the separation and purification of ergosteroside, which is applied in the field of separation and purification of natural ergosteroside to achieve large processing capacity, simple separation and purification process, and good regeneration performance

Inactive Publication Date: 2010-09-22
WUHAN POLYTECHNIC UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is aimed at the defect of existing natural ergosteroside industrial methods, and provides a kind of separation and purification method of natural ergosteroside, and it can obtain the high-purity ergosteroside product by using Budhimonica japonica as raw material; The separation and purification process is simple and convenient , large processing capacity, suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The method for separating and purifying natural ergosteroside of the present invention adopts the following process steps:

[0021] 1. Extraction: Extract the Magnolia flower with ethanol, the extraction conditions are: the mass percentage of ethanol water is 60%, the mass ratio of the raw material of Magnolia flower to the ethanol aqueous solution is 1:6, the extraction temperature is 60℃, and the extraction is carried out twice. The extraction time is 4 hours each time, the extracts are combined, and the extracts of Mongolica are concentrated to dryness under vacuum conditions, which is the crude ergosteroside extract;

[0022] 2. The first step is silica gel column chromatography separation and purification: the crude ergosteroside extract is dissolved in methanol to 1mg / ml, the silica gel: sample solution 1:1 is mixed with the sample, and the ratio of the mixed silica gel to the sample is 1:1. Silica gel chromatography column, ethyl acetate: methanol: water (20:1:0.05-5:...

Embodiment 2

[0029] The method for separating and purifying natural ergosteroside of the present invention adopts the following process steps:

[0030] 1. Extraction: Extract the dense flowers with ethanol, the extraction conditions are: the mass percentage of ethanol water is 95%, the ratio of the raw materials of the dense flowers to the ethanol aqueous solution is 1:10, the extraction temperature is 65 ℃, and the extraction is 3 times, each time The extraction time is 2h, and the extracts are combined, and the extracts of Mongolica are concentrated to dryness under vacuum conditions, which is the crude ergosteroside extract;

[0031] 2. The first step is silica gel column chromatography separation and purification: the crude ergosteroside extract is dissolved in methanol to 1mg / ml, silica gel: sample solution 2:1 mix the sample, pass through a silica gel column, acetic acid chloroform: methanol: water (6:1:0.05-1:1:0.05) Gradient elution, by controlling the pressure, the elution flow rate is...

Embodiment 3

[0037] The method for separating and purifying natural ergosteroside of the present invention adopts the following process steps:

[0038] 1. Extraction: Extract the Magnolia flower with ethanol, the extraction conditions are: the mass percentage of ethanol is 80%, the ratio of raw materials of Magnolia flower to the ethanol aqueous solution is 1:20, the extraction temperature is 50 ℃, and the extraction is 4 times, each extraction The time is 1h, the extracts are combined, and the extracts of Mongolica are concentrated to dryness under vacuum conditions, which is the crude ergosteroside extract;

[0039] 2. The first step is silica gel column chromatography separation and purification: the crude ergosteroside extract is dissolved in methanol to 1mg / ml, silica gel: sample solution 4:1, and the sample is passed through a chromatography column equipped with silica gel, ethyl acetate: methanol: Water (20:1:0.05-5:1:0.05) gradient elution, by controlling the pressure, control the eluti...

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PUM

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Abstract

The invention relates to a method for separating and purifying natural acteoside, and particularly belongs to the technical field of extraction and deep processing. The method is characterized by comprising the following steps of: selecting natural pale butterflybush flower as a raw material, and performing alcohol-containing solvent extraction, silica gel chromatography, polyamide chromatography, macroporous resin absorption and desorption and vacuum drying on the raw material to obtain high-purity natural acteoside. The purity of the extracted acteoside can reach over 90 percent; the separation process is simple and convenient, has large treatment amount, and is suitable for industrialized production; and the method provides a technical support for deeply developing products with high additional values for Chinese medicaments, and provides a natural good-quality raw material for cosmetics, functional food and medicinal industry.

Description

Technical field [0001] The invention belongs to the technical field of extraction and deep processing of Chinese medicinal plants, and is specifically a method for separating and purifying natural ergosteroside. Background technique [0002] Acteoside (Acteoside) is one of the main active ingredients of Chinese herbal medicines such as Dendrolimia, Cistanche, Echinacea. It has a wide range of pharmacological effects, including: 1. Anti-aging effects. Free radicals mediate the oxidation of biological tissues and are related to many pathologies, cancers and aging. This product inhibits the oxidative hemolysis of red blood cells, and its effect is equivalent to that of propyl gallate. Anti-hypoxia effect. It is effective for KCN-induced hypoxia, prolonging survival time by 141%. It has a scavenging effect on superoxide anions, and the SC50 (the concentration required to eliminate half) is 0.063mM. Anti-lipid peroxidation IC 50 It is 3.2μM. 2. Antiviral effect. 3. Antibacteria...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/18C07H1/08
Inventor 陈新聂晗
Owner WUHAN POLYTECHNIC UNIVERSITY
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