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Novel technique for extracting L-tryptophan from fermentation broth

A technology for tryptophan and fermentation broth, applied in the field of biochemical engineering, can solve the problems of low extraction yield, easy product deterioration, environmental pollution, etc., and achieve the effects of improving extraction yield, thorough sterilization and impurity removal, and short filtration time.

Active Publication Date: 2011-12-14
HENAN JULONG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the problems of easy deterioration of the product, low extraction yield and serious environmental pollution in the industrialization process of producing L-tryptophan by the existing fermentation method, and provides a method for separating and extracting L-tryptophan from the fermentation liquid. craft

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The first step is to pretreat the L-tryptophan-containing fermentation broth produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to produce treatment fluid;

[0025] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99....

Embodiment 2

[0033] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;

[0034] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99...

Embodiment 3

[0042] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;

[0043] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top washing with purified water until the tryptophan content in the dope is lower than 1.0g / L, then shut down. Obtain tryptophan microfiltrate and bacterial protein from which the bacteria were removed; the bacteria removal rate reached 99.74%, and the yield of L-tryptophan was 99.3%;

[0044] The third s...

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Abstract

The invention discloses a novel technique for extracting L-tryptophan from fermentation broth, which includes the following steps that: fermentation broth is pretreated, and the treated liquid is pumped into a ceramic microfiltration membrane filter, so that tryptophan microfiltrate without mycelium and mycoprotein are obtained; the tryptophan microfiltrate is pumped into an ultrafiltration membrane filter, so that tryptophan ultrafiltrate and concentrated liquid containing pigment and protein are obtained; the tryptophan ultrafiltrate is pumped into a nanofiltration membrane filter, so that concentrated tryptophan liquid and permeate containing salt are obtained; waste filtrate is comprehensively utilized to produce protein feed; industrial ethylic acid is used for promoting low-temperature crystallization, crystallization mother liquid is treated by the ion exchange method and used, and after crystals are centrifugally dehydrated and dried, the L-tryptophan product is obtained. The invention solves the problem on how to recover industrial ethylic acid, and greatly increases the extraction rate of L-tryptophan; and since the filtrate and the mycoprotein are comprehensively utilized, the product profit is maximized. The total extraction rate of L-tryptophan is 85.2 percent, and the refined product meets the standards of Chinese Pharmacopoeia edition 2005.

Description

Technical field: [0001] The invention relates to the field of biochemical engineering, in particular to a process for separating and extracting L-tryptophan from fermentation broth. Background technique: [0002] According to the calculation of the Food and Agriculture Organization of the United Nations in 2000, the annual demand for L-tryptophan in the world market is about 1.2 million tons or more. In order to promote the application of L-tryptophan, L-tryptophan, whose price has always been relatively high, hopes to have a cheap and efficient production and extraction method. Therefore, the market gap of L-tryptophan is very large and the prospect is bright. The scientific name of L-tryptophan is β-indolylalanine, the chemical name is L-2-amino-3-indolylpropionic acid, alias L-trypsin amino acid, L-aminoindolepropionic acid, molecular formula C 11 h 12 o 2 N 2 , relative molecular mass 204.21. L-Tryptophan is one of the eight essential amino acids in human and anima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/20A23K1/00
Inventor 赵春光邓立君左善孟宗盘
Owner HENAN JULONG BIOLOGICAL ENG CO LTD
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