Novel technique for extracting L-tryptophan from fermentation broth
A technology for tryptophan and fermentation broth, applied in the field of biochemical engineering, can solve the problems of low extraction yield, easy product deterioration, environmental pollution, etc., and achieve the effects of improving extraction yield, thorough sterilization and impurity removal, and short filtration time.
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Embodiment 1
[0024] The first step is to pretreat the L-tryptophan-containing fermentation broth produced by the direct fermentation method: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to produce treatment fluid;
[0025] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99....
Embodiment 2
[0033] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;
[0034] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top wash with purified water until the tryptophan content in the concentrated liquid is lower than 1.0g / L, then stop the machine, and obtain the tryptophan microfiltrate and bacterial protein from which the bacteria are removed. The bacteria removal rate reaches 99.74%, and the L-tryptophan The yield is 99...
Embodiment 3
[0042] The first step, the fermentation broth containing L-tryptophan produced by the direct fermentation method is pretreated: the treatment method is to add 0.1% alum and 0.1% NaHSO in the volume of the fermentation broth, stir and heat to 60 ° C for 30 minutes to prepare treatment fluid;
[0043] The second step is to pump the treatment liquid into the ceramic microfiltration membrane filter, control the temperature at ±45°C, the pressure difference between the membrane inlet and outlet pressure is 0.2~0.3MPa, and the filtration rate is controlled at 2~3m 3 / h, when the clear liquid flow rate is less than 2m 3 / h, divided into several times, each time pumping 1.5 ~ 3m 3 Top washing with purified water until the tryptophan content in the dope is lower than 1.0g / L, then shut down. Obtain tryptophan microfiltrate and bacterial protein from which the bacteria were removed; the bacteria removal rate reached 99.74%, and the yield of L-tryptophan was 99.3%;
[0044] The third s...
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