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Method for identifying cordyceps sinensis powder through microscopic dyeing

A technology for Cordyceps sinensis powder and pure Cordyceps sinensis powder, which is applied in the field of medicinal material identification, can solve the problems of inability to distinguish counterfeit products, inability to detect impurities, and difficulty in distinguishing authenticity, etc., and achieves the effects of protecting benefits, low cost, and not easy to interfere.

Active Publication Date: 2012-11-28
QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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  • Application Information

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Problems solved by technology

[0003] The pure Cordyceps sinensis powder that is currently circulated and sold in the market is made into a certain dosage form, so it is difficult to distinguish the authenticity from the fake, although the means of testing equipment can be used to prove the existence of some markers of Cordyceps sinensis, such as the content of cordycepin and adenosine , but these markers are easy to buy, and they are not expensive, and they can be used as additives in theory, so physical and chemical tests can neither detect impurities nor distinguish fakes

Method used

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  • Method for identifying cordyceps sinensis powder through microscopic dyeing
  • Method for identifying cordyceps sinensis powder through microscopic dyeing
  • Method for identifying cordyceps sinensis powder through microscopic dyeing

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Embodiment Construction

[0032] 5. Experimental methods and parameters:

[0033] 1. Experimental samples:

[0034] Cordyceps slices, commercially available Jicao brand pure Cordyceps superfine powder, starch superfine powder, starch superfine powder and pure Cordyceps superfine powder mixture.

[0035] 2. Dyeing agent

[0036] Fast green dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; safranin dyeing solution: Sinopharm Group Chemical Reagent Co., Ltd.; eosin dyeing solution: Shenyang Xinguang Chemical Factory; hematoxylin dyeing solution: Shenyang Xinguang Chemical Factory; Steiner's solution: Beijing Chemical plant; iodine: Nanjing Xinhuayuan Chemical Co., Ltd., prepared dye solution according to conventional methods.

[0037] 3. Instruments or other utensils

[0038] Binocular biological microscope, camera adapter, Canon SLR camera. Alcohol lamps, glass slides, coverslips, staining racks, cedar oil, absolute ethanol, xylene, Canadian gum, lens cleaning paper, distilled water and ot...

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Abstract

The invention belongs to the medicinal material identifying field and in particular relates to a method for identifying cordyceps sinensis powder through microscopic dyeing. The invention aims to solve the technical problem that the sold pure cordyceps sinensis powder in market is difficult to identify. The technical scheme of the invention used for solving the problem is to provide a method for accurately identifying cordyceps sinensis powder. The method comprises the following steps: taking cordyceps sinensis powder to be detected, dying to prepare smears; placing the prepared smears under a microscope for microscopic observation, and if the special structure of cordyceps sinensis can be clearly observed on each smear without obvious blue spots caused by iodine staining and no obvious plant issue can be observed, judging that the sample is pure cordyceps sinensis powder; and if not, judging that the sample is not pure cordyceps sinensis powder. The method of the invention can be used to clearly identify fine cordyceps sinensis powder prepared for decoction and substances not belonging to cordyceps sinensis, the cost is low, the efficiency is high, method can be not easily interfered by various additives, the accuracy is very high, and the method has good application prospects in the medicinal material identifying field.

Description

technical field [0001] The invention belongs to the field of identification of medicinal materials, and in particular relates to a method for identifying Cordyceps sinensis powder by microscopic staining Background technique [0002] In the traditional medicine of our country, Cordyceps sinensis has always been a high-grade tonic, and its medicinal status is very prominent. It is also known as the "three major tonics" together with ginseng and velvet antler. With the in-depth research in various related disciplines, there have been reports of new species and new distributions of Cordyceps in recent years. Confirmed the efficacy of Cordyceps sinensis. Due to the special growth environment, special geographical environment and important economic value of Cordyceps sinensis, people's research interest is more intense, especially the research on Cordyceps sinensis and its substitutes is very active. [0003] The pure Cordyceps sinensis powder that is currently circulated and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
Inventor 张雪峰徐丽
Owner QINGHAI SPRING MEDICINAL RESOURCES TECHNOLOGY CO LTD
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