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Method for separating and purifying effective ingredients in natural product

A technology of natural products and active ingredients, applied in chemical instruments and methods, semi-permeable membrane separation, ion exchange, etc., can solve the problems of complex process, difficult control, complex components, etc., and achieve optimized process parameters, strong selectivity, extraction, etc. high rate effect

Inactive Publication Date: 2010-08-11
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the complexity of the components in the natural product extract, obtaining the target components by a single membrane separation technology or adsorption separation technology often fails to meet the requirements. Therefore, other separation technologies have to be used, such as precipitation, salting out, and extraction. , crystallization and recrystallization, etc., the process is complex, especially the addition of organic solvents is difficult to handle, and the cost is high
The publication number is CN 1490066, which proposes a method for extracting active ingredients in natural products combining supercritical titanium dioxide fluid extraction technology and molecular distillation technology. It is high investment cost and difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Mix 15g of star anise powder with 750mL of distilled water (or 30% ethanol solution), and extract the active ingredient shikimic acid in star anise at 80°C by hot reflux extraction. The extraction time is 1 hour, the extraction times are 3 times, and suction filtration get the extract. Then use membrane separation technology to remove impurities, select the ultrafiltration membrane with a relative molecular weight cut-off of 10,000 to remove heat sources, polysaccharides, water-soluble proteins, etc., and filter shikimic acid (relative molecular weight is 162). For the above separated and purified extract, activated carbon is used as a solid adsorbent to absorb shikimic acid therein. Adsorption of shikimic acid by activated carbon is an exothermic process, and its operating temperature is 30°C. Then use 30% ethanol solution to desorb the saturated activated carbon. The desorption rate can reach 96.34%.

Embodiment 2

[0018] 15g puerarin powder and 600mL mass concentration of 20% ethanol solution were presoaked for 30 minutes, and the puerarin was extracted by microwave extraction method. The process conditions of microwave extraction were power 1000W, extraction temperature 60°C, extraction time 10 minutes, extraction The number of times is 1 time. Then use membrane separation technology to remove impurities, select ultrafiltration membranes with a relative molecular weight cut-off of 10,000 for purification, intercept and remove heat sources, polysaccharides, water-soluble proteins, etc., and filter puerarin (relative molecular weight 416.38). The total flavonoids can be purified from the retentate obtained by ultrafiltration. The macroporous adsorption resin AB-8 is then used as a solid adsorbent to absorb the puerarin in the filtrate. The adsorption of puerarin by macroporous adsorption resin AB-8 is an exothermic process, and the selected operating temperature is 30°C. Then use 70% e...

Embodiment 3

[0020] Mix 15g of Smilax smilax powder with 450mL of 80% ethanol solution and extract the total flavonoids in Smilax smilax by microwave extraction. The extraction process conditions are power 600W, extraction temperature 80°C, extraction time 5 minutes, and extraction times twice. Firstly, the membrane separation technology is used to remove impurities, and the ultrafiltration membrane with a molecular weight cut-off of 50,000 is selected for purification, and macromolecular proteins, polysaccharides, etc. are intercepted and removed, and total flavonoids can be filtered. The filtrate is subjected to the second membrane separation and purification, and an ultrafiltration membrane with a relative molecular weight cut-off of 6000 is selected to remove aqueous salts and metal ions. The obtained retentate utilizes the macroporous adsorption resin AB-8 as a solid adsorbent to absorb the total flavonoids in the filtrate. Then use the ethanol solution with a mass concentration of 9...

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Abstract

The invention relates to a method for separating and purifying effective ingredients in a natural product. The method comprises the following steps: 1) extracting effective ingredients of the natural product; 2) removing impurities through membrane separation; and 3) purifying through adsorption. The beneficial effects of the invention are as follows: 1) the separation and purification technological processes are considered from an overall perspective, thus making up the defects of membrane separation in the aspect; 2) compared with the traditional 'water extraction and alcohol precipitation' method, the method of the invention uses water as solvent or adds a certain proportion of ethanol according to the characteristics of effective ingredients of the natural product; 3) compared with the ultra-low temperature freezing technology, the method does not adopt the ultra-low temperature state and is more convenient; and 4) compared with the supercritical or subcritical solvent extraction, continuous operation can be realized at room temperature without phase transition course, the technology is simple and is not harsh to devices, the extraction rate is higher, and the final product has high purity.

Description

technical field [0001] The invention belongs to the technical field of chemical separation, and in particular relates to a method for separating and purifying active ingredients in natural products by adopting membrane separation and active carbon adsorption purification technology. Background technique [0002] The content of active ingredients in natural products is often low, and the composition is relatively complex, and the chemical composition of natural products is an important source for the discovery of new drugs or pharmaceutically active lead compounds, and is also widely used in agriculture, industry, daily chemical industry, food and other industries. Therefore, new methods, technologies and processes for the separation and purification of active ingredients in natural products have also been developed. The publication number is CN1919221, which discloses that the traditional method of "water extraction and alcohol precipitation" is used to extract the active in...

Claims

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Application Information

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IPC IPC(8): B01D11/02B01D61/00B01D15/08
Inventor 郭嘉张虎极罗晔黄坤池汝安
Owner WUHAN INSTITUTE OF TECHNOLOGY
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