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Magnetic targeting carrier capable of carrying gene and drug, preparation method and application thereof

A carrier and drug technology, which is applied to a magnetic targeting carrier that can carry genes and drugs and its preparation and application fields, can solve the problems of low expression efficiency and host immune response, etc.

Inactive Publication Date: 2012-01-18
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] At present, the expression efficiency of therapeutic DNA carried by most non-viral vectors is mostly lower than 5%. Secondly, the presence of a large number of non-host DNA expression products derived from vectors, especially CpG sequences, may trigger host immune responses

Method used

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  • Magnetic targeting carrier capable of carrying gene and drug, preparation method and application thereof
  • Magnetic targeting carrier capable of carrying gene and drug, preparation method and application thereof
  • Magnetic targeting carrier capable of carrying gene and drug, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Add 5.2 g of CTAC cationic surfactant and 1.2 g of P123 anionic surfactant to 7.72 ml of tetraethyl orthosilicate ethylene glycol solvent, and 20 g of hydrochloric acid solution at PH=2, mix well and stir at 3000 RPM for 30 hours , add 5ml of concentrated ammonia water, stir at high speed for 5 minutes, and then age for two days, filter, dry at room temperature and sinter at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.

Embodiment 2

[0062] Add 4.6 grams of CTAB cationic surfactant and 2.3 grams of F127 anionic surfactant to ethanol solvent containing 8.15ml tetraethyl orthosilicate, and 20 grams of hydrochloric acid solution with pH=2.5, mix well and stir at 3000RPM for 30 hours at high speed, add 5ml of concentrated ammonia water was stirred at high speed for 5 minutes, aged for two days, filtered, dried at room temperature and sintered at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.

Embodiment 3

[0064] Add 4.9 grams of CTAB cationic surfactant and 1.7 grams of P123 anionic surfactant to a solvent containing 10.22 ml of tetraethyl orthosilicate isopropanol, and 20 grams of hydrochloric acid solution at pH=2, mix well and stir at 3000 RPM for 30 hours , add 5ml of concentrated ammonia water, stir at high speed for 5 minutes, and then age for two days, filter, dry at room temperature and sinter at 700 degrees to obtain an intermediate product A with a mesoporous structure material for use.

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Abstract

The invention discloses a magnetic targeting carrier capable of carrying genes and drugs, a preparation method and an application thereof. The invention is a carrier which has stability, safety and targeting and has controlled release behavior for non-viral magnetic gene therapy and drug therapy. The carrier material of the invention is characterized in that the carrier material is a bunchy silica mesoporous material with magnetism; the length-diameter ratio is not less than 3; the loading capability is big; the material has a protective effect on loaded genes and carriers and superparamagnetism, is not easy to agglomerate, and can control release speed of genes and drugs in vitro; and the surface thereof is easy to modify various functional groups, thus having wide adaptability. The invention also provides a preparation method of the carrier. When in use, therapeutic short chain DNA, siRNA or drugs enter in holes or are combined with surface modified functional genes by a soaking mode, then reach a targeted tissue by guidance of an applied magnetic field, and release the short chain DNA, siRNA or drugs carried thereby under the action of an alternating magnetic field, thus achieving the purpose of magnetic targeting controlled therapy.

Description

technical field [0001] The present invention relates to the fields of biomedicine and nanotechnology, in particular to a carrier with a mesoporous structure and magnetic nanomaterials, DNA carrying therapeutic effects, siRNA and drugs are targeted and concentrated under the induction of an external magnetic field. After the carrier material enters the cell, the The carrier material is moved by an external alternating magnetic field, thereby accelerating the release of DNA, siRNA and drugs. Background technique: [0002] Gene therapy: the use of molecular biology methods to introduce the target gene into the patient's body, so that it can express the target gene product, so that the disease can be treated. Gene therapy is a high-tech-intensive field of biomedicine, which applies the latest research results of molecular biology, molecular genetics, molecular virology, cell biology and other disciplines. Although the technology of gene therapy http: / / www.shenmeshi.com / Science / ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/04A61K47/02A61K47/24A61K48/00
Inventor 徐慧张鹏华
Owner CENT SOUTH UNIV
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