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Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip

A technology using electrochemical and microfluidic chips, applied in measurement devices, scientific instruments, material resistance, etc., to achieve the effects of easy integration and miniaturization, simple equipment, real-time in-vivo detection promotion and maintenance

Inactive Publication Date: 2010-07-28
BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV +2
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Problems solved by technology

At present, the methods of bacterial electrical impedance detection based on microfluidic chips and immunoassays at home and abroad are still in the exploratory and experimental stage.

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  • Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip
  • Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip
  • Method for detecting bacteria by using electrochemical impedance principle and microfluidic chip

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Embodiment Construction

[0026] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0027] Such as figure 1 As shown, the present invention provides a microfluidic chip that can be applied to the electrochemical impedance spectroscopy method. The microfluidic chip includes a microchannel 1, and one end of the microchannel 1 is provided with a sample inlet 11, and the other end is provided with a microfluidic chip. There are 12 sample outlets. The height of the microchannel 1 is 200 μm; the width of the two ends of the microchannel 1 is 300-400 μm, and the length is not limited; the width of the middle section is 600-800 μm, and the length is not limited. A conductive electrode 2 is integrated on the inner bottom surface of the middle section of the microchannel 1 . The conductive electrode 2 is connected with an impedance analyzer 4 through the wire 3, and the change of the electrochemical property of the electrode interface is con...

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Abstract

The invention relates to a method for detecting bacteria by using the electrochemical impedance principle and a microfluidic chip. The method comprises the following steps of 1) arranging one microfluidic chip and determining the functional relation between the system impedance value and the bacterial concentration by using a standard; 2) filling bacteria samples to be detected in a sample inlet of the microfluidic chip and flushing to remove the redundant samples; 3) connecting an impedance analyzer with a conductive electrode in a microfluidic chip detecting zone, reading data displayed in the impedance analyzer and recording the measured impedance value of the bacteria sample; 4) calculating the number of the target bacteria in the bacteria sample according to the determined functionalrelation of the system impedance value and the bacterial concentration. The complex processing for the samples, particularly for the anaerobic bacteria samples, is unnecessary, the required equipmentis simple, the detection is quick, the cost is saved, the integration and miniaturization are easy, and the invention creates favorable conditions for realizing individualization of bacteria detection and real-time in vivo detection of the bacteria so as to better promote and maintain the human health.

Description

technical field [0001] The invention relates to a method and a device for detecting bacteria, in particular to a method for detecting bacteria using the principle of electrochemical impedance and a microfluidic chip. Background technique [0002] Electrochemical impedance spectroscopy (abbreviated as EIS) was called AC impedance in early literature. Impedance measurement was originally a method for studying the frequency response characteristics of linear circuit networks in electricity. In the process of being referred to the study of electrodes, it became an experimental method for electrochemical research, which was introduced in some famous monographs. [0003] Electrochemical impedance spectroscopy is an electrochemical measurement method that uses a small-amplitude sine wave potential (or current) as a disturbance signal. Since the system is perturbed by an electrical signal with a small amplitude, on the one hand, it can avoid a large impact on the system, and on the...

Claims

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Application Information

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IPC IPC(8): G01N27/06G01N27/327
Inventor 裴振华朱涛施生根熊春阳韩秀欣温颖
Owner BEIJING STOMATOLOGY HOSPITAL CAPITAL MEDICAL UNIV
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